Jaw muscle spindle afferents coordinate multiple orofacial motoneurons via common premotor neurons in rats: An electrophysiological and anatomical study

Jingdong Zhang, Pifu Luo, Jin Y. Ro, Huangui Xiong

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Jaw muscle spindle afferents (JMSA) in the mesencephalic trigeminal nucleus (Vme) project to the parvocellular reticular nucleus (PCRt) and dorsomedial spinal trigeminal nucleus (dm-Vsp). A number of premotor neurons that project to the trigeminal motor nucleus (Vmo), facial nucleus (VII) and hypoglossal nucleus (XII) are also located in the PCRt and dm-Vsp. In this study, we examined whether these premotor neurons serve as common relay pool for relaying JMSA to multiple orofacial motoneurons. JMSA inputs to the PCRt and dm-Vsp neurons were verified by recording extracellular responses to electrical stimulation of the caudal Vme or masseter nerve, mechanical stimulation of jaw muscles and jaw opening. After recording, biocytin in recording electrode was inotophorized into recording sites. Biocytin-Iabeled fibers traveled to the Vmo, VII, XII, and the nucleus ambiguus (Amb). Labeled boutons were seen in close apposition with Nissl-stained motoneurons in the Vmo, VII, XII and Amb. In addition, an anterograde tracer (biotinylated dextran amine) was iontophorized into the caudal Vme, and a retrograde tracer (Cholera toxin B subunit) was delivered into either the VII or Xll to identify VII and XII premotor neurons that receive JMSA input. Contacts between labeled Vme neuronal boutons and premotor neurons were observed in the PCRt and adjacent dm-Vsp. Confocal microscopic observations confirmed close contacts between Vme boutons and VII and XII premotor neurons. This study provides evidence that JMSA may coordinate activities of multiple orofacial motor nuclei, including Vmo, VII, XII and Amb in the brainstem via a common premotor neuron pool.

Original languageEnglish (US)
Pages (from-to)37-47
Number of pages11
JournalBrain Research
Volume1489
DOIs
StatePublished - Dec 13 2012

Fingerprint

Muscle Spindles
Motor Neurons
Jaw
Neurons
Tegmentum Mesencephali
Spinal Trigeminal Nucleus
Mediodorsal Thalamic Nucleus
Medulla Oblongata
Cholera Toxin
Electric Stimulation
Brain Stem
Electrodes
Muscles

Keywords

  • Brainstem reticular formation interneurons
  • Orofacial motor nuclei
  • Sensorimotor integration
  • Trigeminal proprioception

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology

Cite this

Jaw muscle spindle afferents coordinate multiple orofacial motoneurons via common premotor neurons in rats : An electrophysiological and anatomical study. / Zhang, Jingdong; Luo, Pifu; Ro, Jin Y.; Xiong, Huangui.

In: Brain Research, Vol. 1489, 13.12.2012, p. 37-47.

Research output: Contribution to journalArticle

@article{6219b42df0624813a23916fe0a631464,
title = "Jaw muscle spindle afferents coordinate multiple orofacial motoneurons via common premotor neurons in rats: An electrophysiological and anatomical study",
abstract = "Jaw muscle spindle afferents (JMSA) in the mesencephalic trigeminal nucleus (Vme) project to the parvocellular reticular nucleus (PCRt) and dorsomedial spinal trigeminal nucleus (dm-Vsp). A number of premotor neurons that project to the trigeminal motor nucleus (Vmo), facial nucleus (VII) and hypoglossal nucleus (XII) are also located in the PCRt and dm-Vsp. In this study, we examined whether these premotor neurons serve as common relay pool for relaying JMSA to multiple orofacial motoneurons. JMSA inputs to the PCRt and dm-Vsp neurons were verified by recording extracellular responses to electrical stimulation of the caudal Vme or masseter nerve, mechanical stimulation of jaw muscles and jaw opening. After recording, biocytin in recording electrode was inotophorized into recording sites. Biocytin-Iabeled fibers traveled to the Vmo, VII, XII, and the nucleus ambiguus (Amb). Labeled boutons were seen in close apposition with Nissl-stained motoneurons in the Vmo, VII, XII and Amb. In addition, an anterograde tracer (biotinylated dextran amine) was iontophorized into the caudal Vme, and a retrograde tracer (Cholera toxin B subunit) was delivered into either the VII or Xll to identify VII and XII premotor neurons that receive JMSA input. Contacts between labeled Vme neuronal boutons and premotor neurons were observed in the PCRt and adjacent dm-Vsp. Confocal microscopic observations confirmed close contacts between Vme boutons and VII and XII premotor neurons. This study provides evidence that JMSA may coordinate activities of multiple orofacial motor nuclei, including Vmo, VII, XII and Amb in the brainstem via a common premotor neuron pool.",
keywords = "Brainstem reticular formation interneurons, Orofacial motor nuclei, Sensorimotor integration, Trigeminal proprioception",
author = "Jingdong Zhang and Pifu Luo and Ro, {Jin Y.} and Huangui Xiong",
year = "2012",
month = "12",
day = "13",
doi = "10.1016/j.brainres.2012.10.021",
language = "English (US)",
volume = "1489",
pages = "37--47",
journal = "Brain Research",
issn = "0006-8993",
publisher = "Elsevier",

}

TY - JOUR

T1 - Jaw muscle spindle afferents coordinate multiple orofacial motoneurons via common premotor neurons in rats

T2 - An electrophysiological and anatomical study

AU - Zhang, Jingdong

AU - Luo, Pifu

AU - Ro, Jin Y.

AU - Xiong, Huangui

PY - 2012/12/13

Y1 - 2012/12/13

N2 - Jaw muscle spindle afferents (JMSA) in the mesencephalic trigeminal nucleus (Vme) project to the parvocellular reticular nucleus (PCRt) and dorsomedial spinal trigeminal nucleus (dm-Vsp). A number of premotor neurons that project to the trigeminal motor nucleus (Vmo), facial nucleus (VII) and hypoglossal nucleus (XII) are also located in the PCRt and dm-Vsp. In this study, we examined whether these premotor neurons serve as common relay pool for relaying JMSA to multiple orofacial motoneurons. JMSA inputs to the PCRt and dm-Vsp neurons were verified by recording extracellular responses to electrical stimulation of the caudal Vme or masseter nerve, mechanical stimulation of jaw muscles and jaw opening. After recording, biocytin in recording electrode was inotophorized into recording sites. Biocytin-Iabeled fibers traveled to the Vmo, VII, XII, and the nucleus ambiguus (Amb). Labeled boutons were seen in close apposition with Nissl-stained motoneurons in the Vmo, VII, XII and Amb. In addition, an anterograde tracer (biotinylated dextran amine) was iontophorized into the caudal Vme, and a retrograde tracer (Cholera toxin B subunit) was delivered into either the VII or Xll to identify VII and XII premotor neurons that receive JMSA input. Contacts between labeled Vme neuronal boutons and premotor neurons were observed in the PCRt and adjacent dm-Vsp. Confocal microscopic observations confirmed close contacts between Vme boutons and VII and XII premotor neurons. This study provides evidence that JMSA may coordinate activities of multiple orofacial motor nuclei, including Vmo, VII, XII and Amb in the brainstem via a common premotor neuron pool.

AB - Jaw muscle spindle afferents (JMSA) in the mesencephalic trigeminal nucleus (Vme) project to the parvocellular reticular nucleus (PCRt) and dorsomedial spinal trigeminal nucleus (dm-Vsp). A number of premotor neurons that project to the trigeminal motor nucleus (Vmo), facial nucleus (VII) and hypoglossal nucleus (XII) are also located in the PCRt and dm-Vsp. In this study, we examined whether these premotor neurons serve as common relay pool for relaying JMSA to multiple orofacial motoneurons. JMSA inputs to the PCRt and dm-Vsp neurons were verified by recording extracellular responses to electrical stimulation of the caudal Vme or masseter nerve, mechanical stimulation of jaw muscles and jaw opening. After recording, biocytin in recording electrode was inotophorized into recording sites. Biocytin-Iabeled fibers traveled to the Vmo, VII, XII, and the nucleus ambiguus (Amb). Labeled boutons were seen in close apposition with Nissl-stained motoneurons in the Vmo, VII, XII and Amb. In addition, an anterograde tracer (biotinylated dextran amine) was iontophorized into the caudal Vme, and a retrograde tracer (Cholera toxin B subunit) was delivered into either the VII or Xll to identify VII and XII premotor neurons that receive JMSA input. Contacts between labeled Vme neuronal boutons and premotor neurons were observed in the PCRt and adjacent dm-Vsp. Confocal microscopic observations confirmed close contacts between Vme boutons and VII and XII premotor neurons. This study provides evidence that JMSA may coordinate activities of multiple orofacial motor nuclei, including Vmo, VII, XII and Amb in the brainstem via a common premotor neuron pool.

KW - Brainstem reticular formation interneurons

KW - Orofacial motor nuclei

KW - Sensorimotor integration

KW - Trigeminal proprioception

UR - http://www.scopus.com/inward/record.url?scp=84869506803&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84869506803&partnerID=8YFLogxK

U2 - 10.1016/j.brainres.2012.10.021

DO - 10.1016/j.brainres.2012.10.021

M3 - Article

C2 - 23085474

AN - SCOPUS:84869506803

VL - 1489

SP - 37

EP - 47

JO - Brain Research

JF - Brain Research

SN - 0006-8993

ER -