Isolation of virulent Yersinia enterocolitica from porcine tongues

M. P. Doyle, M. B. Hugdahl, Stephen L Taylor

Research output: Contribution to journalArticle

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Abstract

Thirty-one tongues from apparently normal, freshly slaughtered pigs were assayed for the presence of Yersinia enterocolitica by different enrichment and postenrichment techniques. Sixteen different isolates were recovered, including six of serotype O:8, four of serotype O:6,30, two of serotype O:3 phage type IXb, and one each of serotypes O:13,7, O:18, and O:46. One isolate was not typable. Cold enrichment in phosphate-buffered saline followed by treatment with dilute KOH or subsequent enrichment in modified Rappaport broth recovered 12 and 7 isolates, respectively. Four of the same isolates were recovered from the same tongues by both procedures. Cold enrichment without a selective postenrichment treatment recovered two isolates. Direct enrichment in modified Rappaport broth or modified selenite broth was ineffective in recovering yersiniae, as no isolates were obtained by either method. All of the serotype O:8 isolates were virulent to mice, causing the death of adults after oral challenge. This is the first report that associates Y. enterocolitica serotype O:8 with a natural reservoir.

Original languageEnglish (US)
Pages (from-to)661-666
Number of pages6
JournalApplied and environmental microbiology
Volume42
Issue number4
StatePublished - Dec 1 1981

Fingerprint

Yersinia enterocolitica
tongue
Tongue
serotypes
Swine
swine
selenite
Selenious Acid
Yersinia
selenites
pig
bacteriophages
Bacteriophages
phosphate
Serogroup
mouth
Phosphates
phosphates
death
mice

ASJC Scopus subject areas

  • Biotechnology
  • Food Science
  • Applied Microbiology and Biotechnology
  • Ecology

Cite this

Isolation of virulent Yersinia enterocolitica from porcine tongues. / Doyle, M. P.; Hugdahl, M. B.; Taylor, Stephen L.

In: Applied and environmental microbiology, Vol. 42, No. 4, 01.12.1981, p. 661-666.

Research output: Contribution to journalArticle

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