Isolation of the phycodnavirus PBCV-1 by biological laser printing

Lisa A. Fitzgerald, Peter K. Wu, James R. Gurnon, Justin C. Biffinger, Bradley R. Ringeisen, James L. Van Etten

Research output: Contribution to journalArticle

10 Scopus citations

Abstract

The Phycodnaviridae family of viruses is diverse genetically but similar morphologically. These viruses infect eukaryotic algal hosts from both fresh and marine waters, and are an important component of aqueous environments. They play important roles in the dynamics of algal blooms, nutrient cycling, algal community structure, and possibly gene transfer between organisms. As such, it is important to identify new viruses within the Phycodnaviridae family. Biological laser printing (BioLP) was used to isolate single virus particles from solution. BioLP prints droplets containing a single virus particle directly onto a host medium, thereby enabling viruses to be isolated from unmodified samples. This manuscript demonstrates how BioLP can be used as a single-step method to separate and possibly identify viruses from complex environmental specimens.

Original languageEnglish (US)
Pages (from-to)223-225
Number of pages3
JournalJournal of Virological Methods
Volume167
Issue number2
DOIs
Publication statusPublished - Aug 1 2010

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Keywords

  • Biological laser printing (BioLP)
  • Chlorella NC64A
  • Chlorella virus PBCV-1
  • Phycodnaviridae

ASJC Scopus subject areas

  • Virology

Cite this

Fitzgerald, L. A., Wu, P. K., Gurnon, J. R., Biffinger, J. C., Ringeisen, B. R., & Van Etten, J. L. (2010). Isolation of the phycodnavirus PBCV-1 by biological laser printing. Journal of Virological Methods, 167(2), 223-225. https://doi.org/10.1016/j.jviromet.2010.04.005