Isolation of human herpesvirus‐6 from clinical specimens using human fibroblast cultures

J. Luka, M. Okano, Geoffrey Milton Thiele

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

The isolation and characterization of human herpesvirus‐6 (HHV‐6) has been hindered by the lack of cell lines useful for its rapid propagation. Recently, we have reported that the MRC‐5 cell line (human diploid lung fibroblasts) was susceptible for HHV‐6 infection. In this study, we report on the isolation of HHV‐6 from the peripheral blood or buffy coat of three chronic fatique syndrome patients, one post‐liver transplant patient, and one severe chronic active Epstein‐Barr virus syndrome patient using the MRC‐5 cell line. Additionally, it was observed by Southern blot hybridization studies that four of five isolates had different restriction enzyme fragment patterns than the isolate obtained from the National Institutes of Health with Eco RI. These data suggest the usefulness of the MRC‐5 cell line in the isolation and characterization of HHV‐6 from various patients.

Original languageEnglish (US)
Pages (from-to)483-486
Number of pages4
JournalJournal of Clinical Laboratory Analysis
Volume4
Issue number6
DOIs
StatePublished - Jan 1 1990

Fingerprint

Fibroblasts
Cell culture
Cells
Cell Line
Deoxyribonuclease EcoRI
Blood Buffy Coat
Transplants
Viruses
National Institutes of Health (U.S.)
Southern Blotting
Diploidy
Blood
Health
Enzymes
Lung
Infection

Keywords

  • Chronic fatigue syndrome
  • herpesvirus
  • liver transplantation
  • peripheral blood cells
  • virus isolation

ASJC Scopus subject areas

  • Immunology and Allergy
  • Hematology
  • Public Health, Environmental and Occupational Health
  • Clinical Biochemistry
  • Medical Laboratory Technology
  • Biochemistry, medical
  • Microbiology (medical)

Cite this

Isolation of human herpesvirus‐6 from clinical specimens using human fibroblast cultures. / Luka, J.; Okano, M.; Thiele, Geoffrey Milton.

In: Journal of Clinical Laboratory Analysis, Vol. 4, No. 6, 01.01.1990, p. 483-486.

Research output: Contribution to journalArticle

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