Dried leaves of Savoy Chieftain cabbage (Brassica oleracea) were extracted with methanol, methylene chloride, or petroleum ether. Soluble extracts were fractionated by flash chromatography on silica gel columns. Nonacosane, 15-nonacosanone, pheophytin a, and β-sitosterol were isolated and examined for their ability to inhibit the mutagenicity of N-methyl-N-nitrosourea (MNU) and 2-aminoanthracene (2-AA) in the Ames bacterial and V79 cell mammalian mutagenicity assays. In the Ames assay, nonacosane and pheophytin were without inhibitory action against either 2-AA- or MNU-induced mutagenesis. 15-Nonacosanone was more effective than 0-sitosterol. In the V79 assay, all four compounds were active against the mutagenicity of 2-AA but only nonacosane, 15-nonacosanone, and 0-sitosterol were active against that of MNU. Other crude extracts were identified with antimutagenic activity, but their compositions were not determined.
ASJC Scopus subject areas
- Agricultural and Biological Sciences(all)