Isolation of Compounds with Antimutagenic Activity from Savoy Chieftain Cabbage

Terence Lawson, Janet Nunnallv, Betty Walker, Edward Bresnick, Desmond Wheeler, Maureen Wheeler

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Dried leaves of Savoy Chieftain cabbage (Brassica oleracea) were extracted with methanol, methylene chloride, or petroleum ether. Soluble extracts were fractionated by flash chromatography on silica gel columns. Nonacosane, 15-nonacosanone, pheophytin a, and β-sitosterol were isolated and examined for their ability to inhibit the mutagenicity of N-methyl-N-nitrosourea (MNU) and 2-aminoanthracene (2-AA) in the Ames bacterial and V79 cell mammalian mutagenicity assays. In the Ames assay, nonacosane and pheophytin were without inhibitory action against either 2-AA- or MNU-induced mutagenesis. 15-Nonacosanone was more effective than 0-sitosterol. In the V79 assay, all four compounds were active against the mutagenicity of 2-AA but only nonacosane, 15-nonacosanone, and 0-sitosterol were active against that of MNU. Other crude extracts were identified with antimutagenic activity, but their compositions were not determined.

Original languageEnglish (US)
Pages (from-to)1363-1367
Number of pages5
JournalJournal of Agricultural and Food Chemistry
Volume37
Issue number5
DOIs
StatePublished - Sep 1 1989

Fingerprint

Brassica oleracea var. sabauda
antimutagenic activity
sitosterols
mutagenicity
Brassica
Assays
assays
Pheophytins
N-methyl-N-nitrosourea
Methylnitrosourea
Mutagenesis
Methylene Chloride
Silica Gel
extracts
methylene chloride
silica gel
Brassica oleracea
Chromatography
Complex Mixtures
mutagenesis

ASJC Scopus subject areas

  • Chemistry(all)
  • Agricultural and Biological Sciences(all)

Cite this

Lawson, T., Nunnallv, J., Walker, B., Bresnick, E., Wheeler, D., & Wheeler, M. (1989). Isolation of Compounds with Antimutagenic Activity from Savoy Chieftain Cabbage. Journal of Agricultural and Food Chemistry, 37(5), 1363-1367. https://doi.org/10.1021/jf00089a034

Isolation of Compounds with Antimutagenic Activity from Savoy Chieftain Cabbage. / Lawson, Terence; Nunnallv, Janet; Walker, Betty; Bresnick, Edward; Wheeler, Desmond; Wheeler, Maureen.

In: Journal of Agricultural and Food Chemistry, Vol. 37, No. 5, 01.09.1989, p. 1363-1367.

Research output: Contribution to journalArticle

Lawson, T, Nunnallv, J, Walker, B, Bresnick, E, Wheeler, D & Wheeler, M 1989, 'Isolation of Compounds with Antimutagenic Activity from Savoy Chieftain Cabbage', Journal of Agricultural and Food Chemistry, vol. 37, no. 5, pp. 1363-1367. https://doi.org/10.1021/jf00089a034
Lawson, Terence ; Nunnallv, Janet ; Walker, Betty ; Bresnick, Edward ; Wheeler, Desmond ; Wheeler, Maureen. / Isolation of Compounds with Antimutagenic Activity from Savoy Chieftain Cabbage. In: Journal of Agricultural and Food Chemistry. 1989 ; Vol. 37, No. 5. pp. 1363-1367.
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