Isolation of black creek canal virus, a new hantavirus from Sigmodon hispidus in Florida

Pierre E. Rollin, Thomas G. Ksiazek, Luanne H. Elliott, Eugene V. Ravkov, Mary Lane Martin, Sergey Morzunov, Walter Livingstone, Marty Monroe, Gregory Glass, Sup Ruo, Ali S Khan, James E. Childs, Stuart T. Nichol, C. J. Peters

Research output: Contribution to journalArticle

119 Citations (Scopus)

Abstract

Numerous rodents were trapped for serologic and virologic studies following the identification of a hantavirus pulmonary syndrome (HPS) case in Dade County, Florida. Cotton rats (Sigmodon hispidus) were the most frequently captured rodent and displayed the highest seroprevalence to a variety of hantavirus antigens. Hantavirus genome RNA was detected in all the seropositive cotton rats tested, using a reverse transcriptase‐polymerase chain reaction (RT‐PCR) assay. A virus was isolated from tissues of two seropositive cotton rats by cultivation of lung and spleen homogenates on Vero E6 cells. Nucleotide sequence information obtained by direct RT‐PCR and the serologic relationships of this virus with the other hantaviruses indicate that this virus, Black Creek Canal virus, represents a new hantavirus distinct from the previously known serotypes. © 1995 Wiley‐Liss, Inc.

Original languageEnglish (US)
Pages (from-to)35-39
Number of pages5
JournalJournal of Medical Virology
Volume46
Issue number1
DOIs
StatePublished - Jan 1 1995

Fingerprint

Sigmodontinae
Hantavirus
Viruses
Rodentia
Hantavirus Pulmonary Syndrome
Vero Cells
Seroepidemiologic Studies
Spleen
Genome
RNA
Antigens
Lung

Keywords

  • Bunyaviridae
  • hantavirus pulmonary syndrome
  • rodent

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

Cite this

Rollin, P. E., Ksiazek, T. G., Elliott, L. H., Ravkov, E. V., Martin, M. L., Morzunov, S., ... Peters, C. J. (1995). Isolation of black creek canal virus, a new hantavirus from Sigmodon hispidus in Florida. Journal of Medical Virology, 46(1), 35-39. https://doi.org/10.1002/jmv.1890460108

Isolation of black creek canal virus, a new hantavirus from Sigmodon hispidus in Florida. / Rollin, Pierre E.; Ksiazek, Thomas G.; Elliott, Luanne H.; Ravkov, Eugene V.; Martin, Mary Lane; Morzunov, Sergey; Livingstone, Walter; Monroe, Marty; Glass, Gregory; Ruo, Sup; Khan, Ali S; Childs, James E.; Nichol, Stuart T.; Peters, C. J.

In: Journal of Medical Virology, Vol. 46, No. 1, 01.01.1995, p. 35-39.

Research output: Contribution to journalArticle

Rollin, PE, Ksiazek, TG, Elliott, LH, Ravkov, EV, Martin, ML, Morzunov, S, Livingstone, W, Monroe, M, Glass, G, Ruo, S, Khan, AS, Childs, JE, Nichol, ST & Peters, CJ 1995, 'Isolation of black creek canal virus, a new hantavirus from Sigmodon hispidus in Florida', Journal of Medical Virology, vol. 46, no. 1, pp. 35-39. https://doi.org/10.1002/jmv.1890460108
Rollin PE, Ksiazek TG, Elliott LH, Ravkov EV, Martin ML, Morzunov S et al. Isolation of black creek canal virus, a new hantavirus from Sigmodon hispidus in Florida. Journal of Medical Virology. 1995 Jan 1;46(1):35-39. https://doi.org/10.1002/jmv.1890460108
Rollin, Pierre E. ; Ksiazek, Thomas G. ; Elliott, Luanne H. ; Ravkov, Eugene V. ; Martin, Mary Lane ; Morzunov, Sergey ; Livingstone, Walter ; Monroe, Marty ; Glass, Gregory ; Ruo, Sup ; Khan, Ali S ; Childs, James E. ; Nichol, Stuart T. ; Peters, C. J. / Isolation of black creek canal virus, a new hantavirus from Sigmodon hispidus in Florida. In: Journal of Medical Virology. 1995 ; Vol. 46, No. 1. pp. 35-39.
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