Isolation of a novel orthobunyavirus from bat flies (Eucampsipoda africana)

Petrus Jansen Van Vuren, Michael R. Wiley, Gustavo Palacios, Nadia Storm, Wanda Markotter, Monica Birkhead, Alan Kemp, Janusz T. Paweska

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

The Bunyaviridae family comprises viruses causing diseases of public and veterinary health importance, including viral haemorrhagic and arboviral fevers. We report the isolation, identification and genome characterization of a novel orthobunyavirus, named Wolkberg virus (WBV), from wingless bat fly ectoparasites (Eucampsipoda africana) of Egyptian fruit bats (Rousettus aegyptiacus) in South Africa. Complete genome sequence data of WBV suggests it is most closely related to two bat viruses (Mojuí dos Campos and Kaeng Khoi viruses) and an arbovirus (Nyando virus) previously shown to infect humans. WBV replicates to high titres in VeroE6 and C6-36 cells, characteristic of mosquito-borne arboviruses. These findings expand our knowledge of the diversity of orthobunyaviruses and their insect vector host range.

Original languageEnglish (US)
Article number000753
Pages (from-to)935-945
Number of pages11
JournalJournal of General Virology
Volume98
Issue number5
DOIs
StatePublished - May 2017

Fingerprint

Orthobunyavirus
Diptera
Viruses
Arboviruses
Viral Hemorrhagic Fevers
Bunyaviridae
Genome
Insect Vectors
Host Specificity
Virus Diseases
South Africa
Culicidae
Fruit
Public Health

Keywords

  • Arbovirus
  • Bat flies
  • Bunyaviridae
  • Egyptian fruit bat
  • Eucampsipoda africana
  • Orthobunyavirus
  • Pathogen discovery
  • Rousettus aegyptiacus

ASJC Scopus subject areas

  • Virology

Cite this

Jansen Van Vuren, P., Wiley, M. R., Palacios, G., Storm, N., Markotter, W., Birkhead, M., ... Paweska, J. T. (2017). Isolation of a novel orthobunyavirus from bat flies (Eucampsipoda africana). Journal of General Virology, 98(5), 935-945. [000753]. https://doi.org/10.1099/jgv.0.000753

Isolation of a novel orthobunyavirus from bat flies (Eucampsipoda africana). / Jansen Van Vuren, Petrus; Wiley, Michael R.; Palacios, Gustavo; Storm, Nadia; Markotter, Wanda; Birkhead, Monica; Kemp, Alan; Paweska, Janusz T.

In: Journal of General Virology, Vol. 98, No. 5, 000753, 05.2017, p. 935-945.

Research output: Contribution to journalArticle

Jansen Van Vuren, P, Wiley, MR, Palacios, G, Storm, N, Markotter, W, Birkhead, M, Kemp, A & Paweska, JT 2017, 'Isolation of a novel orthobunyavirus from bat flies (Eucampsipoda africana)', Journal of General Virology, vol. 98, no. 5, 000753, pp. 935-945. https://doi.org/10.1099/jgv.0.000753
Jansen Van Vuren P, Wiley MR, Palacios G, Storm N, Markotter W, Birkhead M et al. Isolation of a novel orthobunyavirus from bat flies (Eucampsipoda africana). Journal of General Virology. 2017 May;98(5):935-945. 000753. https://doi.org/10.1099/jgv.0.000753
Jansen Van Vuren, Petrus ; Wiley, Michael R. ; Palacios, Gustavo ; Storm, Nadia ; Markotter, Wanda ; Birkhead, Monica ; Kemp, Alan ; Paweska, Janusz T. / Isolation of a novel orthobunyavirus from bat flies (Eucampsipoda africana). In: Journal of General Virology. 2017 ; Vol. 98, No. 5. pp. 935-945.
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