Isolation of a Cellular Subpopulation from a Human Colonic Carcinoma Cell Line

Pamela M. Kimball, Michael G. Brattain

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

A population of cells with increased resistance to 5-fluorouracil was isolated from cultures of the human colonic carcinoma cell line HT29. The resistant cells (HTFU) showed an altered morphology by light and electron microscopy and demonstrated contact inhibition in vitro. DNA assays and chromosome counts showed that HT29 cultures exhibit both hyper-and hypoaneuploidy, while HTFU cultures appear exclusively hypoaneuploid. One year after the isolation of HTFU, both cell lines showed equal sensitivity to 5-fluorodeoxyuridine while HTFU cells retained comparative insensitivity to 5-fluorouracil. Carcinoembryonic antigen production was not demonstrated in pre-or postconfluent cultures of HTFU, although carcinoembryonic antigen was present in both cells and media of HT29 cultures. Growth in semisolid medium was demonstrated for both cultures; however, HTFU showed a lower plating efficiency than did HT29. Tumors were observed in all of the nude mice given injections of HT29 or HTFU cells. Tumors formed from HTFU cells were smaller, and frequently the primary site receded after 6 to 8 weeks. Although in vitro tests suggested a reduced tumorigenic potential for HTFU cells, metastasis was observed only in mice given injections of cells from the HTFU line.

Original languageEnglish (US)
Pages (from-to)1574-1579
Number of pages6
JournalCancer Research
Volume40
Issue number5
StatePublished - May 1 1980

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Carcinoma
Cell Line
Carcinoembryonic Antigen
Fluorouracil
Floxuridine
Contact Inhibition
HT29 Cells
Injections
Nude Mice
Culture Media
Neoplasms
Electron Microscopy
Chromosomes
Neoplasm Metastasis
Light
DNA
Growth
Population
In Vitro Techniques

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Kimball, P. M., & Brattain, M. G. (1980). Isolation of a Cellular Subpopulation from a Human Colonic Carcinoma Cell Line. Cancer Research, 40(5), 1574-1579.

Isolation of a Cellular Subpopulation from a Human Colonic Carcinoma Cell Line. / Kimball, Pamela M.; Brattain, Michael G.

In: Cancer Research, Vol. 40, No. 5, 01.05.1980, p. 1574-1579.

Research output: Contribution to journalArticle

Kimball, PM & Brattain, MG 1980, 'Isolation of a Cellular Subpopulation from a Human Colonic Carcinoma Cell Line', Cancer Research, vol. 40, no. 5, pp. 1574-1579.
Kimball, Pamela M. ; Brattain, Michael G. / Isolation of a Cellular Subpopulation from a Human Colonic Carcinoma Cell Line. In: Cancer Research. 1980 ; Vol. 40, No. 5. pp. 1574-1579.
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