Isolation of a 2-picolinic acid-assimilating bacterium and its proposed degradation pathway

Chunli Zheng, Qiaorui Wang, Yanli Ning, Yurui Fan, Shanshan Feng, Chi He, Tian C Zhang, Zhenxing Shen

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Burkholderia sp. ZD1, aerobically utilizes 2-picolinic acid as a source of carbon, nitrogen and energy, was isolated. ZD1 completely degraded 2-picolinic acid when the initial concentrations ranged from 25 to 300 mg/L. Specific growth rate (μ) and specific consumption rate (q) increased continually in the concentration range of 25–100 mg/L, and then declined. Based on the Haldane model and Andrew's model, μmax and qmax were calculated as 3.9 and 16.5 h−1, respectively. Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR-MS) was used to determine the main intermediates in the degradation pathway. Moreover, attenuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR) was innovatively used to deduce the ring cleavage mechanism of N-heterocycle of 2-picolinic acid. To our knowledge, this is the first report on not only the utilization of 2-picolinic acid by a Burkholderia sp., but also applying FT-ICR-MS and ATR-FTIR for exploring the biodegradation pathway of organic compounds.

Original languageEnglish (US)
Pages (from-to)681-688
Number of pages8
JournalBioresource Technology
Volume245
DOIs
StatePublished - Jan 1 2017

Fingerprint

Bacteria
Degradation
Cyclotron resonance
degradation
bacterium
Acids
acid
FTIR spectroscopy
Fourier transform
Fourier transform infrared spectroscopy
Mass spectrometry
reflectance
Fourier transforms
mass spectrometry
Ions
ion
Biodegradation
Organic compounds
cleavage
organic compound

Keywords

  • 2-Picolinic acid
  • ATR-FTIR
  • Biodegradation pathway
  • Burkholderia sp.
  • FT-ICR-MS

ASJC Scopus subject areas

  • Bioengineering
  • Environmental Engineering
  • Renewable Energy, Sustainability and the Environment
  • Waste Management and Disposal

Cite this

Isolation of a 2-picolinic acid-assimilating bacterium and its proposed degradation pathway. / Zheng, Chunli; Wang, Qiaorui; Ning, Yanli; Fan, Yurui; Feng, Shanshan; He, Chi; Zhang, Tian C; Shen, Zhenxing.

In: Bioresource Technology, Vol. 245, 01.01.2017, p. 681-688.

Research output: Contribution to journalArticle

Zheng, Chunli ; Wang, Qiaorui ; Ning, Yanli ; Fan, Yurui ; Feng, Shanshan ; He, Chi ; Zhang, Tian C ; Shen, Zhenxing. / Isolation of a 2-picolinic acid-assimilating bacterium and its proposed degradation pathway. In: Bioresource Technology. 2017 ; Vol. 245. pp. 681-688.
@article{03b2d83742384108b71e1f8676bff8ae,
title = "Isolation of a 2-picolinic acid-assimilating bacterium and its proposed degradation pathway",
abstract = "Burkholderia sp. ZD1, aerobically utilizes 2-picolinic acid as a source of carbon, nitrogen and energy, was isolated. ZD1 completely degraded 2-picolinic acid when the initial concentrations ranged from 25 to 300 mg/L. Specific growth rate (μ) and specific consumption rate (q) increased continually in the concentration range of 25–100 mg/L, and then declined. Based on the Haldane model and Andrew's model, μmax and qmax were calculated as 3.9 and 16.5 h−1, respectively. Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR-MS) was used to determine the main intermediates in the degradation pathway. Moreover, attenuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR) was innovatively used to deduce the ring cleavage mechanism of N-heterocycle of 2-picolinic acid. To our knowledge, this is the first report on not only the utilization of 2-picolinic acid by a Burkholderia sp., but also applying FT-ICR-MS and ATR-FTIR for exploring the biodegradation pathway of organic compounds.",
keywords = "2-Picolinic acid, ATR-FTIR, Biodegradation pathway, Burkholderia sp., FT-ICR-MS",
author = "Chunli Zheng and Qiaorui Wang and Yanli Ning and Yurui Fan and Shanshan Feng and Chi He and Zhang, {Tian C} and Zhenxing Shen",
year = "2017",
month = "1",
day = "1",
doi = "10.1016/j.biortech.2017.09.031",
language = "English (US)",
volume = "245",
pages = "681--688",
journal = "Bioresource Technology",
issn = "0960-8524",
publisher = "Elsevier Limited",

}

TY - JOUR

T1 - Isolation of a 2-picolinic acid-assimilating bacterium and its proposed degradation pathway

AU - Zheng, Chunli

AU - Wang, Qiaorui

AU - Ning, Yanli

AU - Fan, Yurui

AU - Feng, Shanshan

AU - He, Chi

AU - Zhang, Tian C

AU - Shen, Zhenxing

PY - 2017/1/1

Y1 - 2017/1/1

N2 - Burkholderia sp. ZD1, aerobically utilizes 2-picolinic acid as a source of carbon, nitrogen and energy, was isolated. ZD1 completely degraded 2-picolinic acid when the initial concentrations ranged from 25 to 300 mg/L. Specific growth rate (μ) and specific consumption rate (q) increased continually in the concentration range of 25–100 mg/L, and then declined. Based on the Haldane model and Andrew's model, μmax and qmax were calculated as 3.9 and 16.5 h−1, respectively. Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR-MS) was used to determine the main intermediates in the degradation pathway. Moreover, attenuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR) was innovatively used to deduce the ring cleavage mechanism of N-heterocycle of 2-picolinic acid. To our knowledge, this is the first report on not only the utilization of 2-picolinic acid by a Burkholderia sp., but also applying FT-ICR-MS and ATR-FTIR for exploring the biodegradation pathway of organic compounds.

AB - Burkholderia sp. ZD1, aerobically utilizes 2-picolinic acid as a source of carbon, nitrogen and energy, was isolated. ZD1 completely degraded 2-picolinic acid when the initial concentrations ranged from 25 to 300 mg/L. Specific growth rate (μ) and specific consumption rate (q) increased continually in the concentration range of 25–100 mg/L, and then declined. Based on the Haldane model and Andrew's model, μmax and qmax were calculated as 3.9 and 16.5 h−1, respectively. Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR-MS) was used to determine the main intermediates in the degradation pathway. Moreover, attenuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR) was innovatively used to deduce the ring cleavage mechanism of N-heterocycle of 2-picolinic acid. To our knowledge, this is the first report on not only the utilization of 2-picolinic acid by a Burkholderia sp., but also applying FT-ICR-MS and ATR-FTIR for exploring the biodegradation pathway of organic compounds.

KW - 2-Picolinic acid

KW - ATR-FTIR

KW - Biodegradation pathway

KW - Burkholderia sp.

KW - FT-ICR-MS

UR - http://www.scopus.com/inward/record.url?scp=85029391217&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85029391217&partnerID=8YFLogxK

U2 - 10.1016/j.biortech.2017.09.031

DO - 10.1016/j.biortech.2017.09.031

M3 - Article

VL - 245

SP - 681

EP - 688

JO - Bioresource Technology

JF - Bioresource Technology

SN - 0960-8524

ER -