Isolation and serial propagation of turkey rotaviruses in a fetal rhesus monkey kidney (MA104) cell line.

K. W. Theil, Donald Reynolds, Y. M. Saif

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Turkey rotaviruses from the intestinal contents of poults were isolated and serially propagated in MA104 cell monolayers by a simple procedure. The initial virus isolation was done by low-speed centrifugation of the inoculum onto the monolayers, and subsequent passages were accomplished in roller-tube monolayers using trypsin-treated virus suspensions. Each of the turkey rotavirus isolates possessed the morphologic, antigenic, and genomic attributes characteristic of turkey group A rotaviruses. Attempts to isolate and serially propagate turkey rotavirus-like viruses in MA104 cell monolayers by this procedure were unsuccessful. Turkey reoviruses also did not serially propagate in MA104 cell monolayers by this procedure.

Original languageEnglish (US)
Pages (from-to)93-104
Number of pages12
JournalAvian diseases
Volume30
Issue number1
DOIs
StatePublished - Jan 1 1986

Fingerprint

Rotavirus
kidney cells
Macaca mulatta
cell lines
Kidney
Cell Line
Turkey
Viruses
viruses
Turkey (country)
Gastrointestinal Contents
Reoviridae
poults
cells
Centrifugation
Trypsin
trypsin
centrifugation
Suspensions
inoculum

ASJC Scopus subject areas

  • Food Animals
  • Animal Science and Zoology
  • Immunology and Microbiology(all)

Cite this

Isolation and serial propagation of turkey rotaviruses in a fetal rhesus monkey kidney (MA104) cell line. / Theil, K. W.; Reynolds, Donald; Saif, Y. M.

In: Avian diseases, Vol. 30, No. 1, 01.01.1986, p. 93-104.

Research output: Contribution to journalArticle

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