Isolation and HIV-1 infection of primary human microglia from fetal and adult tissue.

Kathleen Borgmann, Howard Eliot Gendelman, Anuja Ghorpade

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Glial inflammation, principally involving astrocytes and microglia, underlies the pathogenesis of a broad range of neurodegenerative disorders, including, most notably, human immunodeficiency virus (HIV-1)-associated dementia. Indeed, for the latter, disease mechanisms are attributed to viral infection and activation of microglia and perivascular macrophages and their resultant neurotoxic activities. Although monocyte-derived macrophages have served as models for microglia, they are limited both qualitatively and quantitatively in their immune responses and susceptibility to viral infection. Thus, the acquisition of primary human microglial cells is critical for laboratory studies of human neurological disease. In this chapter, we provide detailed methods of isolation, cultivation, characterization, HIV-1 infection, and experimental applications of primary human fetal and adult microglial cells, with particular emphasis on studies of HIV-1 neuropathogenesis.

Original languageEnglish (US)
Pages (from-to)49-70
Number of pages22
JournalMethods in molecular biology (Clifton, N.J.)
Volume304
StatePublished - Jan 1 2005

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Microglia
HIV Infections
HIV-1
Fetus
Virus Diseases
Macrophages
Virus Activation
Neuroglia
Astrocytes
Neurodegenerative Diseases
Dementia
Inflammation

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Isolation and HIV-1 infection of primary human microglia from fetal and adult tissue. / Borgmann, Kathleen; Gendelman, Howard Eliot; Ghorpade, Anuja.

In: Methods in molecular biology (Clifton, N.J.), Vol. 304, 01.01.2005, p. 49-70.

Research output: Contribution to journalArticle

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