Isolation and characterization of the murine transforming growth factor-β2 promoter

Phillip J. Wilder, Michelle M. Desler, Tamara K. Nowling, A Angie Rizzino

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

This report describes the isolation and characterization of the 5′ flanking region of the murine transforming growth factor beta-2 (TGF-β2) gene. A genomic clone containing the promoter region of the gene was isolated after screening a bacteriophage P1 genomic library. The resulting clone was sequenced and compared to promoters for the human and chicken TGF-β2 genes. The sequence located near the transcription start site is highly conserved. It includes a TATA box, an E-box, and a largely conserved CRE/ATF site. A series of murine TGF-β2 promoter/reporter constructs was generated to identify regulatory regions of the gene. As in the case of the human TGF-β2 gene, sequences just upstream of the TATA box, including the CRE/ATF site, actively stimulate the murine TGF-β2 promoter. However, unlike the human TGF-β2 gene, the 5′ flanking region of the murine TGF-β2 gene contains a long alternating purine/pyrimidine repeat that unexpectedly exerts a strong positive effect on its promoter. This is of particular interest since alternating purine/pyrimidine repeats in other promoters have been observed to be inhibitory.

Original languageEnglish (US)
Pages (from-to)201-209
Number of pages9
JournalGene
Volume270
Issue number1-2
DOIs
StatePublished - May 30 2001

Fingerprint

Transforming Growth Factors
Transforming Growth Factor beta
Genes
TATA Box
5' Flanking Region
Clone Cells
Bacteriophage P1
Genomic Library
Nucleic Acid Regulatory Sequences
Transcription Initiation Site
Regulator Genes
Genetic Promoter Regions
Chickens
human TGFB2 protein

Keywords

  • Alternating purine pyrimidine repeat
  • CRE/ATF
  • E-box
  • Embryonal carcinoma cells

ASJC Scopus subject areas

  • Genetics

Cite this

Isolation and characterization of the murine transforming growth factor-β2 promoter. / Wilder, Phillip J.; Desler, Michelle M.; Nowling, Tamara K.; Rizzino, A Angie.

In: Gene, Vol. 270, No. 1-2, 30.05.2001, p. 201-209.

Research output: Contribution to journalArticle

Wilder, Phillip J. ; Desler, Michelle M. ; Nowling, Tamara K. ; Rizzino, A Angie. / Isolation and characterization of the murine transforming growth factor-β2 promoter. In: Gene. 2001 ; Vol. 270, No. 1-2. pp. 201-209.
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