Isolation and characterization of rice mutants compromised in Xa21-mediated resistance to X. oryzae pv. oryzae

G. L. Wang, C. Wu, L. Zeng, C. He, M. Baraoidan, F. De Assis Goes Da Silva, C. E. Williams, P. C. Ronald, H. Leung

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

The rice gene, Xa21, confers resistance to diverse races of Xanthomonas oryzae pv. oryzae (Xoo) and encodes a receptor-like kinase with leucine-rich repeats in the extra-cellular domain. To identify genes essential for the function of the Xa21 gene, 4,500 IRBB21 (Xa21 isogenic line in IR24 background) mutants, induced by diepoxybutane and fast neutrons, were screened against Philippine race six (PR6) Xoo for a change from resistance to susceptibility. From two greenhouse screens, 23 mutants were identified that had changed from resistant to fully (6) or partially (17) susceptible to PR6. All fully susceptible mutants carried rearrangements at the Xa21 locus as detected by PCR and Southern hybridization. For the partially susceptible mutants, no changes were detected at the Xa21 locus based on Southern and PCR analyses. However, two of these mutants were confirmed via genetic analysis to have mutations at the Xa21 locus. Partially susceptible mutants exhibited variation in level of susceptibility to different Xoo strains, suggesting that they may carry different mutations required for the Xa21-mediated resistance. The mutants identified in this study provide useful materials for dissecting the Xa21-mediated resistance pathway in rice.

Original languageEnglish (US)
Pages (from-to)379-384
Number of pages6
JournalTheoretical and Applied Genetics
Volume108
Issue number3
DOIs
StatePublished - Feb 1 2004

Fingerprint

Oryza
rice
mutants
Xanthomonas oryzae pv. oryzae
Xanthomonas
Fast Neutrons
Polymerase Chain Reaction
Mutation
Philippines
Essential Genes
loci
Leucine
Genes
Phosphotransferases
mutation
isogenic lines
Southern blotting
leucine
genetic techniques and protocols
phosphotransferases (kinases)

ASJC Scopus subject areas

  • Biotechnology
  • Agronomy and Crop Science
  • Genetics

Cite this

Isolation and characterization of rice mutants compromised in Xa21-mediated resistance to X. oryzae pv. oryzae. / Wang, G. L.; Wu, C.; Zeng, L.; He, C.; Baraoidan, M.; De Assis Goes Da Silva, F.; Williams, C. E.; Ronald, P. C.; Leung, H.

In: Theoretical and Applied Genetics, Vol. 108, No. 3, 01.02.2004, p. 379-384.

Research output: Contribution to journalArticle

Wang, GL, Wu, C, Zeng, L, He, C, Baraoidan, M, De Assis Goes Da Silva, F, Williams, CE, Ronald, PC & Leung, H 2004, 'Isolation and characterization of rice mutants compromised in Xa21-mediated resistance to X. oryzae pv. oryzae', Theoretical and Applied Genetics, vol. 108, no. 3, pp. 379-384. https://doi.org/10.1007/s00122-003-1452-4
Wang, G. L. ; Wu, C. ; Zeng, L. ; He, C. ; Baraoidan, M. ; De Assis Goes Da Silva, F. ; Williams, C. E. ; Ronald, P. C. ; Leung, H. / Isolation and characterization of rice mutants compromised in Xa21-mediated resistance to X. oryzae pv. oryzae. In: Theoretical and Applied Genetics. 2004 ; Vol. 108, No. 3. pp. 379-384.
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