Isolation and characterization of new wild-type isolates of bovine lentivirus

D. L. Suarez, M. J. VanDerMaaten, Charles Wood, C. A. Whetstone

Research output: Contribution to journalComment/debate

54 Citations (Scopus)

Abstract

Two new isolates of bovine lentivirus, also known as bovine immunodeficiency-like virus (BIV), were obtained from a seropositive cattle herd in Florida. This is the first report of new isolates of BIV since the original BIV strain, R29, was isolated in 1969. The two new BIV isolates were derived from blood buffy coat cells cocultivated in vitro with fetal bovine lung cell cultures. The new isolates differed in vitro from the original R29 isolate in replication and syncytium formation in fetal bovine lung cells. Both new isolates were confirmed as BIV by immunofluorescence assay, Western blotting (immunoblotting), and polymerase chain reaction. Sequence analyses of the polymerase chain reaction pol gene product showed 92.6 and 93.6% homology to the published nucleotide sequence of BIV R29-127, a molecular clone derived from BIV R29. Each of the new BIV isolates was inoculated into two calves, and virus was recovered between 5 and 10 days postinoculation (p.i.), with BIV seroconversion between 10 and 21 days p.i. Virus was recoverable and antibody was detectable for at least 4 months p.i. Two calves developed a transiently elevated mononuclear cell count, similar to what was reported for BIV R29 in the original experimental calf inoculations. No other clinical abnormalities were observed.

Original languageEnglish (US)
Pages (from-to)5051-5055
Number of pages5
JournalJournal of virology
Volume67
Issue number8
StatePublished - Jan 1 1993

Fingerprint

Bovine Lentiviruses
Bovine Immunodeficiency Virus
Lentivirus
immunosuppression
cattle
viruses
Blood Buffy Coat
pol Gene Products
calves
Viruses
Polymerase Chain Reaction
Lung
polymerase chain reaction
lungs
Giant Cells

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

Cite this

Suarez, D. L., VanDerMaaten, M. J., Wood, C., & Whetstone, C. A. (1993). Isolation and characterization of new wild-type isolates of bovine lentivirus. Journal of virology, 67(8), 5051-5055.

Isolation and characterization of new wild-type isolates of bovine lentivirus. / Suarez, D. L.; VanDerMaaten, M. J.; Wood, Charles; Whetstone, C. A.

In: Journal of virology, Vol. 67, No. 8, 01.01.1993, p. 5051-5055.

Research output: Contribution to journalComment/debate

Suarez, DL, VanDerMaaten, MJ, Wood, C & Whetstone, CA 1993, 'Isolation and characterization of new wild-type isolates of bovine lentivirus', Journal of virology, vol. 67, no. 8, pp. 5051-5055.
Suarez DL, VanDerMaaten MJ, Wood C, Whetstone CA. Isolation and characterization of new wild-type isolates of bovine lentivirus. Journal of virology. 1993 Jan 1;67(8):5051-5055.
Suarez, D. L. ; VanDerMaaten, M. J. ; Wood, Charles ; Whetstone, C. A. / Isolation and characterization of new wild-type isolates of bovine lentivirus. In: Journal of virology. 1993 ; Vol. 67, No. 8. pp. 5051-5055.
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