Isolation and characterization of a virus that infects Emiliania huxleyi (Haptophyta)

Tonje Castberg, Runar Thyrhaug, Aud Larsen, Ruth Anne Sandaa, Mikal Heldal, James L. Van Etten, Gunnar Bratbak

Research output: Contribution to journalArticle

68 Scopus citations

Abstract

The isolation and characterization of a virus (designated EhV) that infects the marine coccolithophorid Emiliania huxleyi (Lohmann) Hay & Mohler are described. Three independent clones of EhV were isolated from Norwegian coastal waters in years 1999 and 2000. EhV is a double-stranded DNA-containing virus with a genome size of ∼415 kilo-base pairs. The viral particle is an icosahedron with a diameter of 160-180 nm. The virus particle contains at least nine proteins ranging from 10 to 140 kDa; the major capsid protein weighs ∼54 kDa. EhV has a latent period of 12-14 h and a burst size of 400-1000 (mean, 620) viral particles per cell. A phylogenetic tree based on DNA polymerase amino acid sequences indicates EhV should be assigned to the Phycodnaviridae virus family and that the virus is most closely related to viruses that infect Micromonas pusilla and certain Chlorella species.

Original languageEnglish (US)
Pages (from-to)767-774
Number of pages8
JournalJournal of Phycology
Volume38
Issue number4
DOIs
StatePublished - Aug 2002

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Keywords

  • Algal bloom termination
  • Algal virus
  • Emiliania huxleyi
  • Haptophyta
  • Large dsDNA viruses
  • Phycodnaviridae
  • Virus ecology

ASJC Scopus subject areas

  • Aquatic Science
  • Plant Science

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