Isolation and characterization of a virus from the intracellular green alga symbiotic with Hydra viridis

James L. Van Etten, Russel H. Meints, Dwight E. Burbank, Daniel Kuczmarski, Diane A. Cuppels, Leslie C. Lane

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Abstract

The isolation and characterization of a virus (designated HVCV) from the symbiotic Chlorella-like green alga present in Hydra viridis (Florida strain) are described. The large polyhedral viral particle sedimented at ca. 2600 S in sucrose density gradients and had a buoyant density in CsCl of 1.295 g/ml. The virus contained at least 19 polypeptides (MW range, 10,300 to 82,000) and the major polypeptide (MW, 46,000) was a glycoprotein. The viral genome consisted of a double-stranded DNA with a buoyant density in CsCl of 1.7114 g/ml (52% GC) and a molecular weight of ca. 136 × 106.

Original languageEnglish (US)
Pages (from-to)704-711
Number of pages8
JournalVirology
Volume113
Issue number2
DOIs
StatePublished - Sep 1981

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Hydra
Chlorophyta
Viruses
Chlorella
Peptides
Viral Genome
Virion
Sucrose
Glycoproteins
Molecular Weight
DNA
cesium chloride

ASJC Scopus subject areas

  • Virology

Cite this

Isolation and characterization of a virus from the intracellular green alga symbiotic with Hydra viridis. / Van Etten, James L.; Meints, Russel H.; Burbank, Dwight E.; Kuczmarski, Daniel; Cuppels, Diane A.; Lane, Leslie C.

In: Virology, Vol. 113, No. 2, 09.1981, p. 704-711.

Research output: Contribution to journalArticle

Van Etten, James L. ; Meints, Russel H. ; Burbank, Dwight E. ; Kuczmarski, Daniel ; Cuppels, Diane A. ; Lane, Leslie C. / Isolation and characterization of a virus from the intracellular green alga symbiotic with Hydra viridis. In: Virology. 1981 ; Vol. 113, No. 2. pp. 704-711.
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