Isolation and characterization of a virus from the intracellular green alga symbiotic with Hydra viridis

James L. Van Etten, Russel H. Meints, Dwight E. Burbank, Daniel Kuczmarski, Diane A. Cuppels, Leslie C. Lane

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The isolation and characterization of a virus (designated HVCV) from the symbiotic Chlorella-like green alga present in Hydra viridis (Florida strain) are described. The large polyhedral viral particle sedimented at ca. 2600 S in sucrose density gradients and had a buoyant density in CsCl of 1.295 g/ml. The virus contained at least 19 polypeptides (MW range, 10,300 to 82,000) and the major polypeptide (MW, 46,000) was a glycoprotein. The viral genome consisted of a double-stranded DNA with a buoyant density in CsCl of 1.7114 g/ml (52% GC) and a molecular weight of ca. 136 × 106.

Original languageEnglish (US)
Pages (from-to)704-711
Number of pages8
Issue number2
StatePublished - Sep 1981


ASJC Scopus subject areas

  • Virology

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