Isolation and characterization of a Pti1 homologue from soybean

Ai Guo Tian, Guang Zuo Luo, Yong Jun Wang, Jin Song Zhang, Jun Yi Gai, Shou Yi Chen

Research output: Contribution to journalArticle

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Abstract

A full-length gene GmPti1 was identified from soybean in an EST sequencing project by its homology to tomato Pti1. It encoded a protein of 366 amino acids. RT-PCR analysis showed that the GmPti1 expression was induced by salicylic acid and wounding. The deduced amino acid sequence had a Ser/Thr/Tyr kinase domain. GmPti1 protein was expressed in E. coli as an MBP fusion, purified by amylose resin and examined for its autophosphorylation ability. The phosphorylation assay in vitro showed that GmPti1 had kinase activity in the presence of Mn2+. These results demonstrated that GmPti1 represented a new Pti1-like gene, unlike the two published genes sPti1a and sPti1b, which encoding proteins had no autophosphorylation ability.

Original languageEnglish (US)
Pages (from-to)535-537
Number of pages3
JournalJournal of experimental botany
Volume55
Issue number396
DOIs
StatePublished - Feb 1 2004

Fingerprint

Soybeans
protein phosphorylation
soybeans
phosphotransferases (kinases)
Phosphotransferases
Genes
Amylose
Proteins
Salicylic Acid
genes
proteins
Expressed Sequence Tags
Lycopersicon esculentum
amylose
salicylic acid
resins
Amino Acid Sequence
phosphorylation
amino acid sequences
reverse transcriptase polymerase chain reaction

Keywords

  • Autophosphorylation
  • GmPti1
  • Kinase activity
  • Soybean

ASJC Scopus subject areas

  • Physiology
  • Plant Science

Cite this

Tian, A. G., Luo, G. Z., Wang, Y. J., Zhang, J. S., Gai, J. Y., & Chen, S. Y. (2004). Isolation and characterization of a Pti1 homologue from soybean. Journal of experimental botany, 55(396), 535-537. https://doi.org/10.1093/jxb/erh035

Isolation and characterization of a Pti1 homologue from soybean. / Tian, Ai Guo; Luo, Guang Zuo; Wang, Yong Jun; Zhang, Jin Song; Gai, Jun Yi; Chen, Shou Yi.

In: Journal of experimental botany, Vol. 55, No. 396, 01.02.2004, p. 535-537.

Research output: Contribution to journalArticle

Tian, AG, Luo, GZ, Wang, YJ, Zhang, JS, Gai, JY & Chen, SY 2004, 'Isolation and characterization of a Pti1 homologue from soybean', Journal of experimental botany, vol. 55, no. 396, pp. 535-537. https://doi.org/10.1093/jxb/erh035
Tian, Ai Guo ; Luo, Guang Zuo ; Wang, Yong Jun ; Zhang, Jin Song ; Gai, Jun Yi ; Chen, Shou Yi. / Isolation and characterization of a Pti1 homologue from soybean. In: Journal of experimental botany. 2004 ; Vol. 55, No. 396. pp. 535-537.
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