Isolation and characterization of a Pti1 homologue from soybean

Ai Guo Tian, Guang Zuo Luo, Yong Jun Wang, Jin Song Zhang, Jun Yi Gai, Shou Yi Chen

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Abstract

A full-length gene GmPti1 was identified from soybean in an EST sequencing project by its homology to tomato Pti1. It encoded a protein of 366 amino acids. RT-PCR analysis showed that the GmPti1 expression was induced by salicylic acid and wounding. The deduced amino acid sequence had a Ser/Thr/Tyr kinase domain. GmPti1 protein was expressed in E. coli as an MBP fusion, purified by amylose resin and examined for its autophosphorylation ability. The phosphorylation assay in vitro showed that GmPti1 had kinase activity in the presence of Mn2+. These results demonstrated that GmPti1 represented a new Pti1-like gene, unlike the two published genes sPti1a and sPti1b, which encoding proteins had no autophosphorylation ability.

Original languageEnglish (US)
Pages (from-to)535-537
Number of pages3
JournalJournal of experimental botany
Volume55
Issue number396
DOIs
Publication statusPublished - Feb 1 2004

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Keywords

  • Autophosphorylation
  • GmPti1
  • Kinase activity
  • Soybean

ASJC Scopus subject areas

  • Physiology
  • Plant Science

Cite this

Tian, A. G., Luo, G. Z., Wang, Y. J., Zhang, J. S., Gai, J. Y., & Chen, S. Y. (2004). Isolation and characterization of a Pti1 homologue from soybean. Journal of experimental botany, 55(396), 535-537. https://doi.org/10.1093/jxb/erh035