Isolation and characterization of a gene coding for a novel aspartate aminotransferase from Rhizobium meliloti

J. R. Alfano, M. L. Kahn

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Aspartate aminotransferase (AAT) is an important enzyme in aspartate catabolism and biosynthesis and, by converting tricarboxylic acid cycle intermediates to amino acids, AAT is also significant in linking carbon metabolism with nitrogen metabolism. To examine the role of AAT in symbiotic nitrogen fixation further, plasmids encoding three different aminotransferases from Rhizobium meliloti 104A14 were isolated by complementation of an Escherichia coli auxotroph that lacks three aminotransferases. pJA10 contained a gene, aatB, that coded for a previously undescribed AAT, AatB. pJA30 encoded an aromatic aminotransferase, TatA, that had significant AAT activity, and pJA20 encoded a branched-chain aminotransferase designated BatA. Genes for the latter two enzymes, tatA and batA, were previously isolated from R. meliloti. aatB is distinct from but hybridizes to aatA, which codes for AatA, a protein required for symbiotic nitrogen fixation. The DNA sequence of aatB contained an open reading frame that could encode a protein 410 amino acids long and with a monomer molecular mass of 45,100 Da. The amino acid sequence of aatB is unusual, and AatB appears to be a member of a newly described class of AATs. AatB expressed in E. coli has a K(m) for aspartate of 5.3 mM and a K(m) for 2-oxoglutarate of 0.87 mM. Its pH optimum is between 8.0 and 8.5. Mutations were constructed in aatB and tatA and transferred to the genome of R. meliloti 104A14. Both mutants were prototrophs and were able to carry out symbiotic nitrogen fixation.

Original languageEnglish (US)
Pages (from-to)4186-4196
Number of pages11
JournalJournal of bacteriology
Volume175
Issue number13
DOIs
StatePublished - Jan 1 1993

Fingerprint

Sinorhizobium meliloti
Aspartate Aminotransferases
Nitrogen Fixation
Transaminases
Genes
Aspartic Acid
Escherichia coli
Amino Acids
Citric Acid Cycle
Enzymes
Open Reading Frames
Amino Acid Sequence
Proteins
Plasmids
Nitrogen
Carbon
Genome
Mutation

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

Cite this

Isolation and characterization of a gene coding for a novel aspartate aminotransferase from Rhizobium meliloti. / Alfano, J. R.; Kahn, M. L.

In: Journal of bacteriology, Vol. 175, No. 13, 01.01.1993, p. 4186-4196.

Research output: Contribution to journalArticle

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