Isolation and characterization of a cDNA encoding maize cytosolic malate dehydrogenase

Jian Guan Hu, Xiangshan Zhao, Jun Liu, Zi Qiang Yuan, Jin Shui Yang

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

A cDNA fragment derived from a gene over-expressing in hybrid maize (Zea mays L.) was isolated with RT-PCR and used as probe to screen cDNA library of hybrid maize seedlings. A positive cDNA clone ZH02 corresponding to the full-length mRNA sequence was obtained, which was shown to have an open reading frame encoding 332 a.a. DNA and proteinase database search revealed that the deduced amino acid sequence of ZH02 has high similarity with the cMDH of Mesembryanthemum crystallium L. and Arabidopsis thaliana (L.) Heynh. up to 90% and 84%, respectively. This is the first report of the full-length gene coding for the cereal cMDH.

Original languageEnglish (US)
Pages (from-to)40-44
Number of pages5
JournalActa Botanica Sinica
Volume41
Issue number1
StatePublished - Jan 1 1999

Fingerprint

Malate Dehydrogenase
malate dehydrogenase
Zea mays
Mesembryanthemum
Complementary DNA
Genes
corn
Gene Library
cDNA libraries
open reading frames
Peptide Hydrolases
proteinases
genes
amino acid sequences
Arabidopsis thaliana
reverse transcriptase polymerase chain reaction
Nucleic Acid Databases
clones
Seedlings
Arabidopsis

Keywords

  • Cytosolic malate dehydrogenase
  • Gene cloning
  • Heterosis
  • Maize

ASJC Scopus subject areas

  • Plant Science
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry

Cite this

Hu, J. G., Zhao, X., Liu, J., Yuan, Z. Q., & Yang, J. S. (1999). Isolation and characterization of a cDNA encoding maize cytosolic malate dehydrogenase. Acta Botanica Sinica, 41(1), 40-44.

Isolation and characterization of a cDNA encoding maize cytosolic malate dehydrogenase. / Hu, Jian Guan; Zhao, Xiangshan; Liu, Jun; Yuan, Zi Qiang; Yang, Jin Shui.

In: Acta Botanica Sinica, Vol. 41, No. 1, 01.01.1999, p. 40-44.

Research output: Contribution to journalArticle

Hu, JG, Zhao, X, Liu, J, Yuan, ZQ & Yang, JS 1999, 'Isolation and characterization of a cDNA encoding maize cytosolic malate dehydrogenase', Acta Botanica Sinica, vol. 41, no. 1, pp. 40-44.
Hu, Jian Guan ; Zhao, Xiangshan ; Liu, Jun ; Yuan, Zi Qiang ; Yang, Jin Shui. / Isolation and characterization of a cDNA encoding maize cytosolic malate dehydrogenase. In: Acta Botanica Sinica. 1999 ; Vol. 41, No. 1. pp. 40-44.
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