Isolation and characterization of a-431 cells that retain high epidermal growth factor binding capacity and respond to epidermal growth factor by growth stimulation

A Angie Rizzino, E. Ruff, P. Kazakoff

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

A-431 cells, which exhibit large numbers of epidermal growth factor (EGF) receptors and respond to EGF by growth inhibition, are widely used to study EGF receptors and the effects of EGF. In this report, we describe the isolation and characterization of variant A-431 cells that respond to EGF by growth stimulation. One variant, which is referred to as A-431 R-1, has been characterized in detail. EGF stimulates both monolayer and soft agar growth of A-431 R-1 cells cultured in serum-free medium. In contrast to the original A-431 cells, growth of A-431R-1 cells is not inhibited by EGF, even at high concentrations. Scatchard analysis of EGF binding to A-431R-1 cells and A-431 cells indicates that both cell populations exhibit approximately 1.8 x 104 EGF receptors per cell. Thus, unlike other variants of A-431 cells that are not inhibited by EGF, A-431 R-l cells exhibit as many EGF receptors as the parental A-431 cells. It was also determined that the phorbol ester 12-0-tetradecanoylphorbol-13 acetate reduces EGF binding to the high affinity receptors of A-431 R-l cells; whereas, transforming growth factor type β did not significantly affect EGF binding. Our results suggest that A-431R-1 cells should be useful for studying the biochemical effects of EGF and for examining why some cells are inhibited by EGF, whereas others are stimulated by EGF.

Original languageEnglish (US)
Pages (from-to)2377-2381
Number of pages5
JournalCancer Research
Volume48
Issue number9
StatePublished - May 1988

Fingerprint

Epidermal Growth Factor
Growth
Serum-Free Culture Media
Transforming Growth Factors
Phorbol Esters
Tetradecanoylphorbol Acetate
Agar
Cultured Cells

ASJC Scopus subject areas

  • Medicine(all)
  • Oncology
  • Cancer Research

Cite this

@article{66554de6dda94596bdc895191f07131c,
title = "Isolation and characterization of a-431 cells that retain high epidermal growth factor binding capacity and respond to epidermal growth factor by growth stimulation",
abstract = "A-431 cells, which exhibit large numbers of epidermal growth factor (EGF) receptors and respond to EGF by growth inhibition, are widely used to study EGF receptors and the effects of EGF. In this report, we describe the isolation and characterization of variant A-431 cells that respond to EGF by growth stimulation. One variant, which is referred to as A-431 R-1, has been characterized in detail. EGF stimulates both monolayer and soft agar growth of A-431 R-1 cells cultured in serum-free medium. In contrast to the original A-431 cells, growth of A-431R-1 cells is not inhibited by EGF, even at high concentrations. Scatchard analysis of EGF binding to A-431R-1 cells and A-431 cells indicates that both cell populations exhibit approximately 1.8 x 104 EGF receptors per cell. Thus, unlike other variants of A-431 cells that are not inhibited by EGF, A-431 R-l cells exhibit as many EGF receptors as the parental A-431 cells. It was also determined that the phorbol ester 12-0-tetradecanoylphorbol-13 acetate reduces EGF binding to the high affinity receptors of A-431 R-l cells; whereas, transforming growth factor type β did not significantly affect EGF binding. Our results suggest that A-431R-1 cells should be useful for studying the biochemical effects of EGF and for examining why some cells are inhibited by EGF, whereas others are stimulated by EGF.",
author = "Rizzino, {A Angie} and E. Ruff and P. Kazakoff",
year = "1988",
month = "5",
language = "English (US)",
volume = "48",
pages = "2377--2381",
journal = "Cancer Research",
issn = "0008-5472",
publisher = "American Association for Cancer Research Inc.",
number = "9",

}

TY - JOUR

T1 - Isolation and characterization of a-431 cells that retain high epidermal growth factor binding capacity and respond to epidermal growth factor by growth stimulation

AU - Rizzino, A Angie

AU - Ruff, E.

AU - Kazakoff, P.

PY - 1988/5

Y1 - 1988/5

N2 - A-431 cells, which exhibit large numbers of epidermal growth factor (EGF) receptors and respond to EGF by growth inhibition, are widely used to study EGF receptors and the effects of EGF. In this report, we describe the isolation and characterization of variant A-431 cells that respond to EGF by growth stimulation. One variant, which is referred to as A-431 R-1, has been characterized in detail. EGF stimulates both monolayer and soft agar growth of A-431 R-1 cells cultured in serum-free medium. In contrast to the original A-431 cells, growth of A-431R-1 cells is not inhibited by EGF, even at high concentrations. Scatchard analysis of EGF binding to A-431R-1 cells and A-431 cells indicates that both cell populations exhibit approximately 1.8 x 104 EGF receptors per cell. Thus, unlike other variants of A-431 cells that are not inhibited by EGF, A-431 R-l cells exhibit as many EGF receptors as the parental A-431 cells. It was also determined that the phorbol ester 12-0-tetradecanoylphorbol-13 acetate reduces EGF binding to the high affinity receptors of A-431 R-l cells; whereas, transforming growth factor type β did not significantly affect EGF binding. Our results suggest that A-431R-1 cells should be useful for studying the biochemical effects of EGF and for examining why some cells are inhibited by EGF, whereas others are stimulated by EGF.

AB - A-431 cells, which exhibit large numbers of epidermal growth factor (EGF) receptors and respond to EGF by growth inhibition, are widely used to study EGF receptors and the effects of EGF. In this report, we describe the isolation and characterization of variant A-431 cells that respond to EGF by growth stimulation. One variant, which is referred to as A-431 R-1, has been characterized in detail. EGF stimulates both monolayer and soft agar growth of A-431 R-1 cells cultured in serum-free medium. In contrast to the original A-431 cells, growth of A-431R-1 cells is not inhibited by EGF, even at high concentrations. Scatchard analysis of EGF binding to A-431R-1 cells and A-431 cells indicates that both cell populations exhibit approximately 1.8 x 104 EGF receptors per cell. Thus, unlike other variants of A-431 cells that are not inhibited by EGF, A-431 R-l cells exhibit as many EGF receptors as the parental A-431 cells. It was also determined that the phorbol ester 12-0-tetradecanoylphorbol-13 acetate reduces EGF binding to the high affinity receptors of A-431 R-l cells; whereas, transforming growth factor type β did not significantly affect EGF binding. Our results suggest that A-431R-1 cells should be useful for studying the biochemical effects of EGF and for examining why some cells are inhibited by EGF, whereas others are stimulated by EGF.

UR - http://www.scopus.com/inward/record.url?scp=0023905052&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0023905052&partnerID=8YFLogxK

M3 - Article

C2 - 3258542

AN - SCOPUS:0023905052

VL - 48

SP - 2377

EP - 2381

JO - Cancer Research

JF - Cancer Research

SN - 0008-5472

IS - 9

ER -