Iron(III)-mediated Electrochemical Detection of Levofloxacin in Complex Biological Samples

Hamid R. Lotfi Zadeh Zhad, Rebecca Y. Lai

Research output: Contribution to journalArticle

Abstract

We report the design of an electrochemical sensor capable of detecting levofloxacin (LEVX) in complex biological samples. This detection strategy is simple, fast, and does not require sample pretreatment or electrode modification. Unlike previously developed electrochemical LEVX sensors that require direct oxidation of LEVX, the sensing mechanism is based on the complexation reactions between LEVX and iron(III), resulting in a concentration-dependent decrease in the iron(III) reduction peak current and a shift in the peak potential. These changes are presumably attributed to the decrease in the concentration of uncomplexed Fe(III) in the solution. The concentration-dependent change in both the current and potential can be used for quantification of LEVX in various samples, including 50 % synthetic urine and 25 % synthetic human saliva. The limit of detection was estimated to be in the range of 1.5 to 2.3 μM, concentrations that are much lower than the concentration of LEVX found in urine and saliva samples of patients administered this drug for conditions such as urinary tract infection. With further optimization, this sensing strategy could find applications in clinical pharmacokinetic studies.

Original languageEnglish (US)
Pages (from-to)2672-2677
Number of pages6
JournalElectroanalysis
Volume29
Issue number11
DOIs
StatePublished - Nov 2017

Fingerprint

Electrochemical sensors
Levofloxacin
Iron
Pharmacokinetics
Complexation
Oxidation
Electrodes
Pharmaceutical Preparations

Keywords

  • cyclic voltammetry
  • differential pulse voltammetry
  • ferric chloride
  • levofloxacin
  • square wave voltammetry

ASJC Scopus subject areas

  • Analytical Chemistry
  • Electrochemistry

Cite this

Iron(III)-mediated Electrochemical Detection of Levofloxacin in Complex Biological Samples. / Lotfi Zadeh Zhad, Hamid R.; Lai, Rebecca Y.

In: Electroanalysis, Vol. 29, No. 11, 11.2017, p. 2672-2677.

Research output: Contribution to journalArticle

@article{526d4f35d8494c6e88770a8c0c5278d5,
title = "Iron(III)-mediated Electrochemical Detection of Levofloxacin in Complex Biological Samples",
abstract = "We report the design of an electrochemical sensor capable of detecting levofloxacin (LEVX) in complex biological samples. This detection strategy is simple, fast, and does not require sample pretreatment or electrode modification. Unlike previously developed electrochemical LEVX sensors that require direct oxidation of LEVX, the sensing mechanism is based on the complexation reactions between LEVX and iron(III), resulting in a concentration-dependent decrease in the iron(III) reduction peak current and a shift in the peak potential. These changes are presumably attributed to the decrease in the concentration of uncomplexed Fe(III) in the solution. The concentration-dependent change in both the current and potential can be used for quantification of LEVX in various samples, including 50 {\%} synthetic urine and 25 {\%} synthetic human saliva. The limit of detection was estimated to be in the range of 1.5 to 2.3 μM, concentrations that are much lower than the concentration of LEVX found in urine and saliva samples of patients administered this drug for conditions such as urinary tract infection. With further optimization, this sensing strategy could find applications in clinical pharmacokinetic studies.",
keywords = "cyclic voltammetry, differential pulse voltammetry, ferric chloride, levofloxacin, square wave voltammetry",
author = "{Lotfi Zadeh Zhad}, {Hamid R.} and Lai, {Rebecca Y.}",
year = "2017",
month = "11",
doi = "10.1002/elan.201700428",
language = "English (US)",
volume = "29",
pages = "2672--2677",
journal = "Electroanalysis",
issn = "1040-0397",
publisher = "Wiley-VCH Verlag",
number = "11",

}

TY - JOUR

T1 - Iron(III)-mediated Electrochemical Detection of Levofloxacin in Complex Biological Samples

AU - Lotfi Zadeh Zhad, Hamid R.

AU - Lai, Rebecca Y.

PY - 2017/11

Y1 - 2017/11

N2 - We report the design of an electrochemical sensor capable of detecting levofloxacin (LEVX) in complex biological samples. This detection strategy is simple, fast, and does not require sample pretreatment or electrode modification. Unlike previously developed electrochemical LEVX sensors that require direct oxidation of LEVX, the sensing mechanism is based on the complexation reactions between LEVX and iron(III), resulting in a concentration-dependent decrease in the iron(III) reduction peak current and a shift in the peak potential. These changes are presumably attributed to the decrease in the concentration of uncomplexed Fe(III) in the solution. The concentration-dependent change in both the current and potential can be used for quantification of LEVX in various samples, including 50 % synthetic urine and 25 % synthetic human saliva. The limit of detection was estimated to be in the range of 1.5 to 2.3 μM, concentrations that are much lower than the concentration of LEVX found in urine and saliva samples of patients administered this drug for conditions such as urinary tract infection. With further optimization, this sensing strategy could find applications in clinical pharmacokinetic studies.

AB - We report the design of an electrochemical sensor capable of detecting levofloxacin (LEVX) in complex biological samples. This detection strategy is simple, fast, and does not require sample pretreatment or electrode modification. Unlike previously developed electrochemical LEVX sensors that require direct oxidation of LEVX, the sensing mechanism is based on the complexation reactions between LEVX and iron(III), resulting in a concentration-dependent decrease in the iron(III) reduction peak current and a shift in the peak potential. These changes are presumably attributed to the decrease in the concentration of uncomplexed Fe(III) in the solution. The concentration-dependent change in both the current and potential can be used for quantification of LEVX in various samples, including 50 % synthetic urine and 25 % synthetic human saliva. The limit of detection was estimated to be in the range of 1.5 to 2.3 μM, concentrations that are much lower than the concentration of LEVX found in urine and saliva samples of patients administered this drug for conditions such as urinary tract infection. With further optimization, this sensing strategy could find applications in clinical pharmacokinetic studies.

KW - cyclic voltammetry

KW - differential pulse voltammetry

KW - ferric chloride

KW - levofloxacin

KW - square wave voltammetry

UR - http://www.scopus.com/inward/record.url?scp=85030153861&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85030153861&partnerID=8YFLogxK

U2 - 10.1002/elan.201700428

DO - 10.1002/elan.201700428

M3 - Article

AN - SCOPUS:85030153861

VL - 29

SP - 2672

EP - 2677

JO - Electroanalysis

JF - Electroanalysis

SN - 1040-0397

IS - 11

ER -