Iron-Sulfur Clusters in DNA Polymerases and Primases of Eukaryotes

Andrey G. Baranovskiy, Hollie M. Siebler, Youri I Pavlov, Tahir H Tahirov

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Research during the past decade witnessed the discovery of [4Fe-4S] clusters in several members of the eukaryotic DNA replication machinery. The presence of clusters was confirmed by UV-visible absorption, electron paramagnetic resonance spectroscopy, and metal analysis for primase and the B-family DNA polymerases δ and ζ. The crystal structure of primase revealed that the [4Fe-4S] cluster is buried inside the protein and fulfills a structural role. Although [4Fe-4S] clusters are firmly established in the C-terminal domains of catalytic subunits of DNA polymerases δ and ζ, no structures are currently available and their precise roles have not been ascertained. The [4Fe-4S] clusters in the polymerases and primase play a structural role ensuring proper protein folding and stability. In DNA polymerases δ and ζ, they can potentially play regulatory role by sensing hurdles during DNA replication and assisting with DNA polymerase switches by oscillation between oxidized-reduced states.

Original languageEnglish (US)
JournalMethods in Enzymology
DOIs
StateAccepted/In press - Jan 1 2017

Fingerprint

DNA Primase
DNA-Directed DNA Polymerase
Eukaryota
Sulfur
Iron
DNA Replication
Metal analysis
Catalytic DNA
Protein folding
Protein Stability
DNA
Protein Folding
Electron Spin Resonance Spectroscopy
Machinery
Paramagnetic resonance
Catalytic Domain
Spectrum Analysis
Crystal structure
Metals
Switches

Keywords

  • B-subunit
  • Crystal structure
  • DNA polymerase
  • DNA replication
  • Iron-sulfur cluster
  • Primase

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

Cite this

Iron-Sulfur Clusters in DNA Polymerases and Primases of Eukaryotes. / Baranovskiy, Andrey G.; Siebler, Hollie M.; Pavlov, Youri I; Tahirov, Tahir H.

In: Methods in Enzymology, 01.01.2017.

Research output: Contribution to journalArticle

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