Iron-Sulfur Clusters in DNA Polymerases and Primases of Eukaryotes

Andrey G. Baranovskiy, Hollie M. Siebler, Youri I Pavlov, Tahir H Tahirov

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

Research during the past decade witnessed the discovery of [4Fe-4S] clusters in several members of the eukaryotic DNA replication machinery. The presence of clusters was confirmed by UV-visible absorption, electron paramagnetic resonance spectroscopy, and metal analysis for primase and the B-family DNA polymerases δ and ζ. The crystal structure of primase revealed that the [4Fe-4S] cluster is buried inside the protein and fulfills a structural role. Although [4Fe-4S] clusters are firmly established in the C-terminal domains of catalytic subunits of DNA polymerases δ and ζ, no structures are currently available and their precise roles have not been ascertained. The [4Fe-4S] clusters in the polymerases and primase play a structural role ensuring proper protein folding and stability. In DNA polymerases δ and ζ, they can potentially play regulatory role by sensing hurdles during DNA replication and assisting with DNA polymerase switches by oscillation between oxidized-reduced states.

Original languageEnglish (US)
JournalMethods in Enzymology
DOIs
StateAccepted/In press - Jan 1 2017

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Keywords

  • B-subunit
  • Crystal structure
  • DNA polymerase
  • DNA replication
  • Iron-sulfur cluster
  • Primase

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

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