Ionic Driven Embedment of Hyaluronic Acid Coated Liposomes in Polyelectrolyte Multilayer Films for Local Therapeutic Delivery

Stephen L. Hayward, David M. Francis, Matthew J. Sis, Srivatsan S Kidambi

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

The ability to control the spatial distribution and temporal release of a therapeutic remains a central challenge for biomedical research. Here, we report the development and optimization of a novel substrate mediated therapeutic delivery system comprising of hyaluronic acid covalently functionalized liposomes (HALNPs) embedded into polyelectrolyte multilayer (PEM) platform via ionic stabilization. The PEM platform was constructed from sequential deposition of Poly-LLysine (PLL) and Poly(Sodium styrene sulfonate) (SPS) "(PLL/SPS)4.5" followed by adsorption of anionic HALNPs. An adsorption affinity assay and saturation curve illustrated the preferential HALNP deposition density for precise therapeutic loading. (PLL/SPS)2.5 capping layer on top of the deposited HALNP monolayer further facilitated complete nanoparticle immobilization, cell adhesion, and provided nanoparticle confinement for controlled linear release profiles of the nanocarrier and encapsulated cargo. To our knowledge, this is the first study to demonstrate the successful embedment of a translatable lipid based nanocarrier into a substrate that allows for temporal and spatial release of both hydrophobic and hydrophilic drugs. Specifically, we have utilized our platform to deliver chemotherapeutic drug Doxorubicin from PEM confined HALNPs. Overall, we believe the development of our HALNP embedded PEM system is significant and will catalyze the usage of substrate mediated delivery platforms in biomedical applications.

Original languageEnglish (US)
Article number14683
JournalScientific reports
Volume5
DOIs
StatePublished - Oct 1 2015

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Hyaluronic Acid
Liposomes
Nanoparticles
Adsorption
Styrene
Therapeutics
Cell Adhesion
Immobilization
Pharmaceutical Preparations
Doxorubicin
Biomedical Research
Sodium
Lipids
Polyelectrolytes

ASJC Scopus subject areas

  • General

Cite this

Ionic Driven Embedment of Hyaluronic Acid Coated Liposomes in Polyelectrolyte Multilayer Films for Local Therapeutic Delivery. / Hayward, Stephen L.; Francis, David M.; Sis, Matthew J.; Kidambi, Srivatsan S.

In: Scientific reports, Vol. 5, 14683, 01.10.2015.

Research output: Contribution to journalArticle

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