Ion-neutral collisional cross sections of carbohydrate isomers as divalent cation adducts and their electron transfer products

Yuting Huang, Eric D. Dodds

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

As the gravity of glycoscience continues to amass, a commensurate demand for rapid, sensitive, and structurally comprehensive glycoanalytical tools has arisen. Among the most elusive but desirable analytical capabilities is the ability to expeditiously and unambiguously detect, distinguish, and resolve carbohydrates that differ only in their constitutional isomerism and/or stereoisomerism. While ion mobility spectrometry (IMS) has proven a highly promising tool for such analyses, the facility of this method to discriminate larger oligosaccharides is still somewhat limited. In an effort to expand the capabilities of IMS to discriminate among carbohydrate isomers, the present investigation was focused on IMS studies of four trisaccharide isomers, four pentasaccharide isomers, and two hexasaccharide isomers, each as group II metal ion adducts and their corresponding gas-phase electron transfer (ET) products. These studies were also evaluated in the context of previously investigated group I metal ion adducts of the same saccharides. The orientationally averaged ion-neutral collisional cross sections (CCSs) of the various carbohydrate/metal ion adducts were found to be dependent on the structures of specific carbohydrate isomers, sensitive to the electronic characteristics of the bound cation, and responsive to the attachment of an additional electron (in the case of divalent metal ion adducts). Overall, these results underscore the utility of metal ions for probing carbohydrate structure in concert with IMS, and the capacity of gas-phase ion chemistry to expand the menu of such probes.

Original languageEnglish (US)
Pages (from-to)6912-6921
Number of pages10
JournalAnalyst
Volume140
Issue number20
DOIs
StatePublished - Oct 21 2015

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Divalent Cations
Carbohydrates
Isomers
carbohydrate
cross section
cation
Positive ions
Metal ions
Electrons
Ions
electron
Spectrometry
ion
Metals
spectrometry
Spectrum Analysis
metal
Gases
Trisaccharides
Oligosaccharides

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Environmental Chemistry
  • Spectroscopy
  • Electrochemistry

Cite this

Ion-neutral collisional cross sections of carbohydrate isomers as divalent cation adducts and their electron transfer products. / Huang, Yuting; Dodds, Eric D.

In: Analyst, Vol. 140, No. 20, 21.10.2015, p. 6912-6921.

Research output: Contribution to journalArticle

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