Inward rectifier potassium (Kir) channels in the soybean aphid Aphis glycines: Functional characterization, pharmacology, and toxicology

Peter M. Piermarini, Edna Alfaro Inocente, Nuris Acosta, Corey R Hopkins, Jerod S. Denton, Andrew P. Michel

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Inward rectifier K+ (Kir) channels contribute to a variety of physiological processes in insects and are emerging targets for insecticide development. Previous studies on insect Kir channels have primarily focused on dipteran species (e.g., mosquitoes, fruit flies). Here we identify and functionally characterize Kir channel subunits in a hemipteran insect, the soybean aphid Aphis glycines, which is an economically important insect pest and vector of soybeans. From the transcriptome and genome of Ap. glycines we identified two cDNAs, ApKir1 and ApKir2, encoding Kir subunits that were orthologs of insect Kir1 and Kir2, respectively. Notably, a gene encoding a Kir3 subunit was absent from the transcriptome and genome of Ap. glycines, similar to the pea aphid Acyrthosiphon pisum. Heterologous expression of ApKir1 and ApKir2 in Xenopus laevis oocytes enhanced K+-currents in the plasma membrane; these currents were inhibited by barium and the small molecule VU041. Compared to ApKir2, ApKir1 mediated currents that were larger in magnitude, more sensitive to barium, and less inhibited by small molecule VU041. Moreover, ApKir1 exhibited stronger inward rectification compared to ApKir2. Topical application of VU041 in adult aphids resulted in dose-dependent mortality within 24 h that was more efficacious than flonicamid, an established insecticide also known to inhibit Kir channels. We conclude that despite the apparent loss of Kir3 genes in aphid evolution, Kir channels are important to aphid survival and represent a promising target for the development of new insecticides.

Original languageEnglish (US)
Pages (from-to)57-65
Number of pages9
JournalJournal of Insect Physiology
Volume110
DOIs
StatePublished - Oct 2018

Fingerprint

Inwardly Rectifying Potassium Channel
Aphis glycines
Aphids
potassium channels
toxicology
pharmacology
Soybeans
Toxicology
Glycine
Pharmacology
Insects
Aphidoidea
Insecticides
insects
barium
insecticides
Acyrthosiphon pisum
transcriptome
flonicamid
Barium

Keywords

  • Aphid
  • Barium
  • Insecticide
  • Kir channel
  • Small molecule
  • VU041

ASJC Scopus subject areas

  • Physiology
  • Insect Science

Cite this

Inward rectifier potassium (Kir) channels in the soybean aphid Aphis glycines : Functional characterization, pharmacology, and toxicology. / Piermarini, Peter M.; Inocente, Edna Alfaro; Acosta, Nuris; Hopkins, Corey R; Denton, Jerod S.; Michel, Andrew P.

In: Journal of Insect Physiology, Vol. 110, 10.2018, p. 57-65.

Research output: Contribution to journalArticle

Piermarini, Peter M. ; Inocente, Edna Alfaro ; Acosta, Nuris ; Hopkins, Corey R ; Denton, Jerod S. ; Michel, Andrew P. / Inward rectifier potassium (Kir) channels in the soybean aphid Aphis glycines : Functional characterization, pharmacology, and toxicology. In: Journal of Insect Physiology. 2018 ; Vol. 110. pp. 57-65.
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