Involvement of sigma-1 receptor in astrocyte activation induced by methamphetamine via up-regulation of its own expression

Yuan Zhang, Xuan Lv, Ying Bai, Xinjian Zhu, Xiaodong Wu, Jie Chao, Ming Duan, Shilpa J Buch, Ling Chen, Honghong Yao

Research output: Contribution to journalArticle

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Abstract

Background: Although it has been documented that methamphetamine induces astrocyte activation, the mechanism(s) underlying this effect remain poorly understood. We thus sought to examine the molecular mechanisms involved in methamphetamine-mediated activation of astrocytes with a focus on the role of sigma-1 receptor (σ-1R) in this process. Methods: The expression of σ-1R and glial fibrillary acidic protein (GFAP) was examined by reverse transcription PCR (RT-PCR), real-time PCR, Western blot, and immunofluorescent staining; phosphorylation of cell signaling pathways was detected by Western blot analysis. Immunoprecipitation was used to determine the interaction between σ-1R and p-Src. Chromatin immunoprecipitation (ChIP) assay was employed to discern the binding of cAMP-response element-binding protein (CREB) with the promoter of σ-1R. The role of σ-1R in astrocyte activation was further validated in σ-1R knockout (KO) mice by Western blot combined with immunofluorescent staining. Results: Exposure of primary rat astrocytes to methamphetamine increased the expression of σ-1R via the activation of Src, ERK mitogen-activated protein kinase, and downstream CREB pathways. Subsequently, CREB translocated into nucleus and interacted with the promoter of σ-1R resulting in increased expression of σ-1R with a concomitant increase in expression of GFAP. This effect was inhibited in cells treated with the σ-1R antagonist-BD1047, thereby implicating the role of σ-1R in the activation of astrocytes. In vivo relevance of these findings was further corroborated in σ-1R KO mice that were administered methamphetamine. In the methamphetamine administered mice, there was a failure of the drug to induce activation of astrocytes, an effect that was evident in wild-type (WT) mice exposed to methamphetamine. Conclusions: The study presented herein demonstrates that methamphetamine-mediated activation of astrocytes involved up-regulation of σ-1R through a positive-feedback mechanism. Understanding the regulation of σ-1R expression could provide insights into the development of potential therapeutic strategies for astrocyte activation induced by methamphetamine.

Original languageEnglish (US)
Article number29
JournalJournal of Neuroinflammation
Volume12
Issue number1
DOIs
StatePublished - Dec 12 2015

Fingerprint

Methamphetamine
Astrocytes
Up-Regulation
Cyclic AMP Response Element-Binding Protein
Western Blotting
Glial Fibrillary Acidic Protein
Knockout Mice
Staining and Labeling
sigma-1 receptor
Chromatin Immunoprecipitation
Mitogen-Activated Protein Kinases
Immunoprecipitation
Reverse Transcription
Real-Time Polymerase Chain Reaction
Phosphorylation
Polymerase Chain Reaction
Pharmaceutical Preparations

Keywords

  • Astrocytes
  • CREB
  • ERK
  • Methamphetamine
  • Sigma-1 receptor

ASJC Scopus subject areas

  • Neuroscience(all)
  • Immunology
  • Neurology
  • Cellular and Molecular Neuroscience

Cite this

Involvement of sigma-1 receptor in astrocyte activation induced by methamphetamine via up-regulation of its own expression. / Zhang, Yuan; Lv, Xuan; Bai, Ying; Zhu, Xinjian; Wu, Xiaodong; Chao, Jie; Duan, Ming; Buch, Shilpa J; Chen, Ling; Yao, Honghong.

In: Journal of Neuroinflammation, Vol. 12, No. 1, 29, 12.12.2015.

Research output: Contribution to journalArticle

Zhang, Yuan ; Lv, Xuan ; Bai, Ying ; Zhu, Xinjian ; Wu, Xiaodong ; Chao, Jie ; Duan, Ming ; Buch, Shilpa J ; Chen, Ling ; Yao, Honghong. / Involvement of sigma-1 receptor in astrocyte activation induced by methamphetamine via up-regulation of its own expression. In: Journal of Neuroinflammation. 2015 ; Vol. 12, No. 1.
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AU - Bai, Ying

AU - Zhu, Xinjian

AU - Wu, Xiaodong

AU - Chao, Jie

AU - Duan, Ming

AU - Buch, Shilpa J

AU - Chen, Ling

AU - Yao, Honghong

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