Involvement of mast cells in adenosine-mediated bronchoconstriction and inflammation in an allergic mouse model

Peter J Oldenburg, S. Jamal Mustafa

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

In allergen-induced asthma, activation of lung mast cells leads to bronchial constriction, increased mucus secretion, and an increase in the localization of inflammatory cells to the airways. The purpose of this study was to explore the role of mast cells in adenosine-mediated airway reactivity and inflammation using the mast cell degranulating agent, compound 48/80 (C48/80). Mice were sensitized and challenged with ragweed (or 0.9% saline) followed by C48/80 administration twice a day in increasing doses for 5 days. Dose-responsiveness to the nonspecific adenosine receptor agonist 5′-N-ethylcarboxamidoadenosine (NECA) was established, and lung lavage was performed 24 h later for cell differential analysis to evaluate inflammation. At a dose of 375 μg/ml (aerosolized NECA), C48/80 pretreatment resulted in a significant attenuation in airway reactivity when compared with sensitized control mice (330.07 versus 581.57%, respectively). Lung lavage from the C48/80 treated mice showed a decrease in eosinophils (17.7 versus 60.9%, respectively) and an increase in macrophages when compared with the sensitized control group (76.4 versus 30.8%, respectively). These results support the conclusion that mast cell degranulation plays an important role in adenosine receptor-mediated airway hyperresponsiveness and inflammation.

Original languageEnglish (US)
Pages (from-to)319-324
Number of pages6
JournalJournal of Pharmacology and Experimental Therapeutics
Volume313
Issue number1
DOIs
StatePublished - Apr 1 2005

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p-Methoxy-N-methylphenethylamine
Bronchoconstriction
Mast Cells
Adenosine
Inflammation
Adenosine-5'-(N-ethylcarboxamide)
Bronchoalveolar Lavage
Purinergic P1 Receptor Agonists
Ambrosia
Cell Degranulation
Purinergic P1 Receptors
Mucus
Eosinophils
Allergens
Asthma
Macrophages
Lung
Control Groups

ASJC Scopus subject areas

  • Molecular Medicine
  • Pharmacology

Cite this

Involvement of mast cells in adenosine-mediated bronchoconstriction and inflammation in an allergic mouse model. / Oldenburg, Peter J; Mustafa, S. Jamal.

In: Journal of Pharmacology and Experimental Therapeutics, Vol. 313, No. 1, 01.04.2005, p. 319-324.

Research output: Contribution to journalArticle

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