Involvement of both PKS and NRPS in antibacterial activity in Lysobacter enzymogenes OH11

Juan Zhang, Liangcheng Du, Fengquan Liu, Feifei Xu, Baishi Hu, Vittorio Venturi, Guoliang Qian

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Polyketides and nonribosomal peptides represent two large families of natural products (NPs) with diverse structures and important functions. They are synthesized by polyketide synthase (PKS) and nonribosomal peptide synthetase (NRPS), respectively. Lysobacter enzymogenes is emerging as a novel biocontrol agent against pathogens of crop plants and a new source of bioactive NPs, such as antibacterial antibiotic WAP-8294A2 and antifungal antibiotic HSAF. Genome survey of strain OH11, a Chinese L. enzymogenes isolate, detected four novel PKS, NRPS or hybrid gene clusters, designed as cluster A to D. We further individually mutated five genes (PKS or NRPS) located in these four gene clusters and showed that a PKS gene in cluster A and an NRPS gene in cluster D were involved in the antibacterial activity via a WAP-8294A2 dependent way. The data also showed that none of the five genes was associated with antifungal activity and the regulation of HSAF biosynthesis. Our results reveal the unusual regulatory role of these PKS and NRPS genes that were discovered from genome mining in L. enzymogenes.

Original languageEnglish (US)
Pages (from-to)170-176
Number of pages7
JournalFEMS Microbiology Letters
Volume355
Issue number2
DOIs
StatePublished - Jun 2014

Fingerprint

Lysobacter
Peptide Synthases
Polyketide Synthases
Multigene Family
Biological Products
Genome
Genes
Polyketides
Antifungal Agents
Anti-Bacterial Agents
Peptides

Keywords

  • Lysobacter enzymogenes
  • Nonribosomal peptide synthetase
  • Polyketide synthase
  • WAP-8294A2

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology
  • Genetics

Cite this

Involvement of both PKS and NRPS in antibacterial activity in Lysobacter enzymogenes OH11. / Zhang, Juan; Du, Liangcheng; Liu, Fengquan; Xu, Feifei; Hu, Baishi; Venturi, Vittorio; Qian, Guoliang.

In: FEMS Microbiology Letters, Vol. 355, No. 2, 06.2014, p. 170-176.

Research output: Contribution to journalArticle

Zhang, Juan ; Du, Liangcheng ; Liu, Fengquan ; Xu, Feifei ; Hu, Baishi ; Venturi, Vittorio ; Qian, Guoliang. / Involvement of both PKS and NRPS in antibacterial activity in Lysobacter enzymogenes OH11. In: FEMS Microbiology Letters. 2014 ; Vol. 355, No. 2. pp. 170-176.
@article{1cd56cd029e74f5bb75f736cda611bdf,
title = "Involvement of both PKS and NRPS in antibacterial activity in Lysobacter enzymogenes OH11",
abstract = "Polyketides and nonribosomal peptides represent two large families of natural products (NPs) with diverse structures and important functions. They are synthesized by polyketide synthase (PKS) and nonribosomal peptide synthetase (NRPS), respectively. Lysobacter enzymogenes is emerging as a novel biocontrol agent against pathogens of crop plants and a new source of bioactive NPs, such as antibacterial antibiotic WAP-8294A2 and antifungal antibiotic HSAF. Genome survey of strain OH11, a Chinese L. enzymogenes isolate, detected four novel PKS, NRPS or hybrid gene clusters, designed as cluster A to D. We further individually mutated five genes (PKS or NRPS) located in these four gene clusters and showed that a PKS gene in cluster A and an NRPS gene in cluster D were involved in the antibacterial activity via a WAP-8294A2 dependent way. The data also showed that none of the five genes was associated with antifungal activity and the regulation of HSAF biosynthesis. Our results reveal the unusual regulatory role of these PKS and NRPS genes that were discovered from genome mining in L. enzymogenes.",
keywords = "Lysobacter enzymogenes, Nonribosomal peptide synthetase, Polyketide synthase, WAP-8294A2",
author = "Juan Zhang and Liangcheng Du and Fengquan Liu and Feifei Xu and Baishi Hu and Vittorio Venturi and Guoliang Qian",
year = "2014",
month = "6",
doi = "10.1111/1574-6968.12457",
language = "English (US)",
volume = "355",
pages = "170--176",
journal = "FEMS Microbiology Letters",
issn = "0378-1097",
publisher = "Wiley-Blackwell",
number = "2",

}

TY - JOUR

T1 - Involvement of both PKS and NRPS in antibacterial activity in Lysobacter enzymogenes OH11

AU - Zhang, Juan

AU - Du, Liangcheng

AU - Liu, Fengquan

AU - Xu, Feifei

AU - Hu, Baishi

AU - Venturi, Vittorio

AU - Qian, Guoliang

PY - 2014/6

Y1 - 2014/6

N2 - Polyketides and nonribosomal peptides represent two large families of natural products (NPs) with diverse structures and important functions. They are synthesized by polyketide synthase (PKS) and nonribosomal peptide synthetase (NRPS), respectively. Lysobacter enzymogenes is emerging as a novel biocontrol agent against pathogens of crop plants and a new source of bioactive NPs, such as antibacterial antibiotic WAP-8294A2 and antifungal antibiotic HSAF. Genome survey of strain OH11, a Chinese L. enzymogenes isolate, detected four novel PKS, NRPS or hybrid gene clusters, designed as cluster A to D. We further individually mutated five genes (PKS or NRPS) located in these four gene clusters and showed that a PKS gene in cluster A and an NRPS gene in cluster D were involved in the antibacterial activity via a WAP-8294A2 dependent way. The data also showed that none of the five genes was associated with antifungal activity and the regulation of HSAF biosynthesis. Our results reveal the unusual regulatory role of these PKS and NRPS genes that were discovered from genome mining in L. enzymogenes.

AB - Polyketides and nonribosomal peptides represent two large families of natural products (NPs) with diverse structures and important functions. They are synthesized by polyketide synthase (PKS) and nonribosomal peptide synthetase (NRPS), respectively. Lysobacter enzymogenes is emerging as a novel biocontrol agent against pathogens of crop plants and a new source of bioactive NPs, such as antibacterial antibiotic WAP-8294A2 and antifungal antibiotic HSAF. Genome survey of strain OH11, a Chinese L. enzymogenes isolate, detected four novel PKS, NRPS or hybrid gene clusters, designed as cluster A to D. We further individually mutated five genes (PKS or NRPS) located in these four gene clusters and showed that a PKS gene in cluster A and an NRPS gene in cluster D were involved in the antibacterial activity via a WAP-8294A2 dependent way. The data also showed that none of the five genes was associated with antifungal activity and the regulation of HSAF biosynthesis. Our results reveal the unusual regulatory role of these PKS and NRPS genes that were discovered from genome mining in L. enzymogenes.

KW - Lysobacter enzymogenes

KW - Nonribosomal peptide synthetase

KW - Polyketide synthase

KW - WAP-8294A2

UR - http://www.scopus.com/inward/record.url?scp=84902532964&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84902532964&partnerID=8YFLogxK

U2 - 10.1111/1574-6968.12457

DO - 10.1111/1574-6968.12457

M3 - Article

C2 - 24801439

AN - SCOPUS:84902532964

VL - 355

SP - 170

EP - 176

JO - FEMS Microbiology Letters

JF - FEMS Microbiology Letters

SN - 0378-1097

IS - 2

ER -