Investigations of Rodent Urinary Bladder Carcinogens

Collection, Processing, and Evaluation of Urine and Bladders

Samuel Monroe Cohen, Takamasa Ohnishi, Nicole M. Clark, Jun He, Lora L Arnold

Research output: Contribution to journalArticle

64 Citations (Scopus)

Abstract

Examination of the urine and the bladder epithelium are essential to the investigation of mechanisms of urinary bladder carcinogens in rodents. However, urine and bladder tissue specimens must be collected and processed properly if accurate data are to be derived. The optimum specimen for analysis of urinary constituents is fresh void urine collected from nonfasting animals. Fasting the animal prior to urine collection changes the normal composition, including pH. Many of the normal urinary constituents can influence the mode of action of bladder carcinogens, especially for non-genotoxic agents. Light microscopy is routinely used to examine the bladder epithelium. However, it is often necessary to use more sensitive techniques, such as scanning electron microscopy (SEM) to detect subtle cytotoxic changes in the superficial cell layer of the urothelium, and bromodeoxyuridine (BrdU) incorporation, PCNA, or Ki-67 immunohistochemistry to determine the labeling index for cell proliferation. The urinary bladder must be handled gently and inflated with fixative in situ before the animal dies to avoid artifactual autolytic damage to the bladder epithelium that is visible by SEM and may be mistaken for treatment-related changes. The purpose of this paper is to provide information for the proper collection and examination of urine and the urinary bladder.

Original languageEnglish (US)
Pages (from-to)337-347
Number of pages11
JournalToxicologic Pathology
Volume35
Issue number3
DOIs
StatePublished - Jan 1 2007

Fingerprint

Carcinogens
Rodentia
Animals
Urinary Bladder
Urine
Processing
Fixatives
Scanning electron microscopy
Proliferating Cell Nuclear Antigen
Cell proliferation
Bromodeoxyuridine
Urine Specimen Collection
Labeling
Optical microscopy
Epithelium
Cells
Electron Scanning Microscopy
Tissue
Chemical analysis
Urothelium

Keywords

  • Urinary bladder
  • cell proliferation
  • hyperplasia
  • scanning electron microscopy
  • urine
  • urine collection
  • urothelium

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Molecular Biology
  • Toxicology
  • Cell Biology

Cite this

Investigations of Rodent Urinary Bladder Carcinogens : Collection, Processing, and Evaluation of Urine and Bladders. / Cohen, Samuel Monroe; Ohnishi, Takamasa; Clark, Nicole M.; He, Jun; Arnold, Lora L.

In: Toxicologic Pathology, Vol. 35, No. 3, 01.01.2007, p. 337-347.

Research output: Contribution to journalArticle

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