Investigations of bio markers for human lymphoblastoid cells using atomic force microscopy

Ruiguo Yang, Ning Xi, Carmen Kar Man Fung, King Wai Chiu Lai, Kristina Seiffert-Sinha, Animesh A. Sinha, Weijing Zhang

Research output: Chapter in Book/Report/Conference proceedingConference contribution

4 Citations (Scopus)

Abstract

We report the use of Atomic Force Microcopy (AFM) in combination with Fluorescence Microscopy (FM) for the investigation of non-Hodgkin's type B cells lymphoma after antibody treatment. Rituximab is used in the treatment this cancer type. It is a chimeric monoclonal antibody directed against the protein Cluster of Differentiation 20 (CD20), which is expressed on mature B cells, including the B cell lymphoma surface. We applied anti-CD20 antibody to the B lymphoma cells that was labeled with fluorophore. Simultaneous imaging was then performed by both FM and AFM. Immunofluorescence imaging confirms the binding of Rituximab to the CD20 protein; while the AFM imaging revealed changes in lymphoma cells in terms of mean cell height and cell surface roughness. In addition, our AFM based nanomanipulation system can assist in the analysis of mechanical property of the cell by recording the force-displacement curves at the cell surface. AFM imaging and measurements may provide biomarkers of cell behavior and may facilitate the treatment of lymphoma clinically as a pretest to determine the effectiveness of the drug Rituximab on lymphoma cells from individual patients.

Original languageEnglish (US)
Title of host publication2010 IEEE 5th International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2010
Pages129-132
Number of pages4
DOIs
StatePublished - Nov 29 2010
Event5th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2010 - Xiamen, China
Duration: Jan 20 2010Jan 23 2010

Publication series

Name2010 IEEE 5th International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2010

Other

Other5th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2010
CountryChina
CityXiamen
Period1/20/101/23/10

Fingerprint

Atomic force microscopy
Imaging techniques
Fluorescence microscopy
Cells
Antibodies
Proteins
Monoclonal antibodies
Oncology
Fluorophores
Biomarkers
Surface roughness
Mechanical properties

Keywords

  • AFM live cell imaging
  • Atomic force microscopy
  • Cell immobilization
  • Fluorescence microscopy
  • Human lymphoblastoid cells
  • Rituximab

ASJC Scopus subject areas

  • Control and Systems Engineering
  • Electrical and Electronic Engineering

Cite this

Yang, R., Xi, N., Fung, C. K. M., Lai, K. W. C., Seiffert-Sinha, K., Sinha, A. A., & Zhang, W. (2010). Investigations of bio markers for human lymphoblastoid cells using atomic force microscopy. In 2010 IEEE 5th International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2010 (pp. 129-132). [5592162] (2010 IEEE 5th International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2010). https://doi.org/10.1109/NEMS.2010.5592162

Investigations of bio markers for human lymphoblastoid cells using atomic force microscopy. / Yang, Ruiguo; Xi, Ning; Fung, Carmen Kar Man; Lai, King Wai Chiu; Seiffert-Sinha, Kristina; Sinha, Animesh A.; Zhang, Weijing.

2010 IEEE 5th International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2010. 2010. p. 129-132 5592162 (2010 IEEE 5th International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2010).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Yang, R, Xi, N, Fung, CKM, Lai, KWC, Seiffert-Sinha, K, Sinha, AA & Zhang, W 2010, Investigations of bio markers for human lymphoblastoid cells using atomic force microscopy. in 2010 IEEE 5th International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2010., 5592162, 2010 IEEE 5th International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2010, pp. 129-132, 5th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2010, Xiamen, China, 1/20/10. https://doi.org/10.1109/NEMS.2010.5592162
Yang R, Xi N, Fung CKM, Lai KWC, Seiffert-Sinha K, Sinha AA et al. Investigations of bio markers for human lymphoblastoid cells using atomic force microscopy. In 2010 IEEE 5th International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2010. 2010. p. 129-132. 5592162. (2010 IEEE 5th International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2010). https://doi.org/10.1109/NEMS.2010.5592162
Yang, Ruiguo ; Xi, Ning ; Fung, Carmen Kar Man ; Lai, King Wai Chiu ; Seiffert-Sinha, Kristina ; Sinha, Animesh A. ; Zhang, Weijing. / Investigations of bio markers for human lymphoblastoid cells using atomic force microscopy. 2010 IEEE 5th International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2010. 2010. pp. 129-132 (2010 IEEE 5th International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2010).
@inproceedings{df46fbd9decf49749bf9dd8954249b8b,
title = "Investigations of bio markers for human lymphoblastoid cells using atomic force microscopy",
abstract = "We report the use of Atomic Force Microcopy (AFM) in combination with Fluorescence Microscopy (FM) for the investigation of non-Hodgkin's type B cells lymphoma after antibody treatment. Rituximab is used in the treatment this cancer type. It is a chimeric monoclonal antibody directed against the protein Cluster of Differentiation 20 (CD20), which is expressed on mature B cells, including the B cell lymphoma surface. We applied anti-CD20 antibody to the B lymphoma cells that was labeled with fluorophore. Simultaneous imaging was then performed by both FM and AFM. Immunofluorescence imaging confirms the binding of Rituximab to the CD20 protein; while the AFM imaging revealed changes in lymphoma cells in terms of mean cell height and cell surface roughness. In addition, our AFM based nanomanipulation system can assist in the analysis of mechanical property of the cell by recording the force-displacement curves at the cell surface. AFM imaging and measurements may provide biomarkers of cell behavior and may facilitate the treatment of lymphoma clinically as a pretest to determine the effectiveness of the drug Rituximab on lymphoma cells from individual patients.",
keywords = "AFM live cell imaging, Atomic force microscopy, Cell immobilization, Fluorescence microscopy, Human lymphoblastoid cells, Rituximab",
author = "Ruiguo Yang and Ning Xi and Fung, {Carmen Kar Man} and Lai, {King Wai Chiu} and Kristina Seiffert-Sinha and Sinha, {Animesh A.} and Weijing Zhang",
year = "2010",
month = "11",
day = "29",
doi = "10.1109/NEMS.2010.5592162",
language = "English (US)",
isbn = "9781424465439",
series = "2010 IEEE 5th International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2010",
pages = "129--132",
booktitle = "2010 IEEE 5th International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2010",

}

TY - GEN

T1 - Investigations of bio markers for human lymphoblastoid cells using atomic force microscopy

AU - Yang, Ruiguo

AU - Xi, Ning

AU - Fung, Carmen Kar Man

AU - Lai, King Wai Chiu

AU - Seiffert-Sinha, Kristina

AU - Sinha, Animesh A.

AU - Zhang, Weijing

PY - 2010/11/29

Y1 - 2010/11/29

N2 - We report the use of Atomic Force Microcopy (AFM) in combination with Fluorescence Microscopy (FM) for the investigation of non-Hodgkin's type B cells lymphoma after antibody treatment. Rituximab is used in the treatment this cancer type. It is a chimeric monoclonal antibody directed against the protein Cluster of Differentiation 20 (CD20), which is expressed on mature B cells, including the B cell lymphoma surface. We applied anti-CD20 antibody to the B lymphoma cells that was labeled with fluorophore. Simultaneous imaging was then performed by both FM and AFM. Immunofluorescence imaging confirms the binding of Rituximab to the CD20 protein; while the AFM imaging revealed changes in lymphoma cells in terms of mean cell height and cell surface roughness. In addition, our AFM based nanomanipulation system can assist in the analysis of mechanical property of the cell by recording the force-displacement curves at the cell surface. AFM imaging and measurements may provide biomarkers of cell behavior and may facilitate the treatment of lymphoma clinically as a pretest to determine the effectiveness of the drug Rituximab on lymphoma cells from individual patients.

AB - We report the use of Atomic Force Microcopy (AFM) in combination with Fluorescence Microscopy (FM) for the investigation of non-Hodgkin's type B cells lymphoma after antibody treatment. Rituximab is used in the treatment this cancer type. It is a chimeric monoclonal antibody directed against the protein Cluster of Differentiation 20 (CD20), which is expressed on mature B cells, including the B cell lymphoma surface. We applied anti-CD20 antibody to the B lymphoma cells that was labeled with fluorophore. Simultaneous imaging was then performed by both FM and AFM. Immunofluorescence imaging confirms the binding of Rituximab to the CD20 protein; while the AFM imaging revealed changes in lymphoma cells in terms of mean cell height and cell surface roughness. In addition, our AFM based nanomanipulation system can assist in the analysis of mechanical property of the cell by recording the force-displacement curves at the cell surface. AFM imaging and measurements may provide biomarkers of cell behavior and may facilitate the treatment of lymphoma clinically as a pretest to determine the effectiveness of the drug Rituximab on lymphoma cells from individual patients.

KW - AFM live cell imaging

KW - Atomic force microscopy

KW - Cell immobilization

KW - Fluorescence microscopy

KW - Human lymphoblastoid cells

KW - Rituximab

UR - http://www.scopus.com/inward/record.url?scp=78649277683&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=78649277683&partnerID=8YFLogxK

U2 - 10.1109/NEMS.2010.5592162

DO - 10.1109/NEMS.2010.5592162

M3 - Conference contribution

SN - 9781424465439

T3 - 2010 IEEE 5th International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2010

SP - 129

EP - 132

BT - 2010 IEEE 5th International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2010

ER -