Investigation of a sensing approach based on a rapid reduction of azide to selectively measure bioavailability of H2S

Gunwoo Kim, Eunju Jang, Alexis M. Page, Ting Ding, Kimberly A. Carlson, Haishi Cao

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

A new reaction-based sensor (AHS) was synthesized for quantitative detection of H2S. AHS showed a high selectivity and sensitivity toward H2S over other thio-containing molecules, or reducing reagents with high abundance in living cells. In the presence of H2S, significant fluorescence enhancement (17-fold) was observed due to the reduction of the azide on AHS. The absorption (362 nm) and fluorescence emission (557 nm) of reduced AHS showed a highly linear correlation to H2S level, which were used to measure concentration of H2S in the range of 0-100 μM.

Original languageEnglish (US)
Pages (from-to)95920-95924
Number of pages5
JournalRSC Advances
Volume6
Issue number98
DOIs
StatePublished - Jan 1 2016

Fingerprint

Azides
Fluorescence
Cells
Molecules
Sensors
Biological Availability

ASJC Scopus subject areas

  • Chemistry(all)
  • Chemical Engineering(all)

Cite this

Investigation of a sensing approach based on a rapid reduction of azide to selectively measure bioavailability of H2S. / Kim, Gunwoo; Jang, Eunju; Page, Alexis M.; Ding, Ting; Carlson, Kimberly A.; Cao, Haishi.

In: RSC Advances, Vol. 6, No. 98, 01.01.2016, p. 95920-95924.

Research output: Contribution to journalArticle

@article{7d289fa4e0234b68acd430b30b6e1977,
title = "Investigation of a sensing approach based on a rapid reduction of azide to selectively measure bioavailability of H2S",
abstract = "A new reaction-based sensor (AHS) was synthesized for quantitative detection of H2S. AHS showed a high selectivity and sensitivity toward H2S over other thio-containing molecules, or reducing reagents with high abundance in living cells. In the presence of H2S, significant fluorescence enhancement (17-fold) was observed due to the reduction of the azide on AHS. The absorption (362 nm) and fluorescence emission (557 nm) of reduced AHS showed a highly linear correlation to H2S level, which were used to measure concentration of H2S in the range of 0-100 μM.",
author = "Gunwoo Kim and Eunju Jang and Page, {Alexis M.} and Ting Ding and Carlson, {Kimberly A.} and Haishi Cao",
year = "2016",
month = "1",
day = "1",
doi = "10.1039/c6ra20478c",
language = "English (US)",
volume = "6",
pages = "95920--95924",
journal = "RSC Advances",
issn = "2046-2069",
publisher = "Royal Society of Chemistry",
number = "98",

}

TY - JOUR

T1 - Investigation of a sensing approach based on a rapid reduction of azide to selectively measure bioavailability of H2S

AU - Kim, Gunwoo

AU - Jang, Eunju

AU - Page, Alexis M.

AU - Ding, Ting

AU - Carlson, Kimberly A.

AU - Cao, Haishi

PY - 2016/1/1

Y1 - 2016/1/1

N2 - A new reaction-based sensor (AHS) was synthesized for quantitative detection of H2S. AHS showed a high selectivity and sensitivity toward H2S over other thio-containing molecules, or reducing reagents with high abundance in living cells. In the presence of H2S, significant fluorescence enhancement (17-fold) was observed due to the reduction of the azide on AHS. The absorption (362 nm) and fluorescence emission (557 nm) of reduced AHS showed a highly linear correlation to H2S level, which were used to measure concentration of H2S in the range of 0-100 μM.

AB - A new reaction-based sensor (AHS) was synthesized for quantitative detection of H2S. AHS showed a high selectivity and sensitivity toward H2S over other thio-containing molecules, or reducing reagents with high abundance in living cells. In the presence of H2S, significant fluorescence enhancement (17-fold) was observed due to the reduction of the azide on AHS. The absorption (362 nm) and fluorescence emission (557 nm) of reduced AHS showed a highly linear correlation to H2S level, which were used to measure concentration of H2S in the range of 0-100 μM.

UR - http://www.scopus.com/inward/record.url?scp=84991489607&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84991489607&partnerID=8YFLogxK

U2 - 10.1039/c6ra20478c

DO - 10.1039/c6ra20478c

M3 - Article

AN - SCOPUS:84991489607

VL - 6

SP - 95920

EP - 95924

JO - RSC Advances

JF - RSC Advances

SN - 2046-2069

IS - 98

ER -