Investigating the Effect of Impurities on Macromolecule Crystal Growth in Microgravity

Edward H. Snell, Russell A. Judge, Lisa Crawford, Elizabeth L. Forsythe, Marc L. Pusey, Michael Sportiello, Paul Todd, Henry Bellamy, Jeff Lovelace, John M. Cassanto, Gloria E Borgstahl

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

Chicken egg-white lysozyme (CEWL) crystals were grown in microgravity and on the ground in the presence of various amounts of a naturally occurring lysozyme dimer impurity. No significant favorable differences in impurity incorporation between microgravity and ground crystal samples were observed. At low impurity concentration the microgravity crystals preferentially incorporated the dimer. The presence of the dimer in the crystallization solutions in microgravity reduced crystal size, increased mosaicity, and reduced the signal-to-noise ratio of the X-ray data. Microgravity samples proved more sensitive to impurity. Accurate indexing of the reflections proved critical to the X-ray analysis. The largest crystals with the best X-ray diffraction properties were grown from pure solution in microgravity.

Original languageEnglish (US)
Pages (from-to)151-158
Number of pages8
JournalCrystal Growth and Design
Volume1
Issue number2
DOIs
StatePublished - Mar 1 2001

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Microgravity
Crystallization
microgravity
Macromolecules
macromolecules
Crystal growth
crystal growth
Impurities
impurities
Crystals
Dimers
crystals
lysozyme
dimers
Muramidase
Enzymes
chickens
x rays
eggs
X ray analysis

ASJC Scopus subject areas

  • Chemistry(all)
  • Materials Science(all)
  • Condensed Matter Physics

Cite this

Snell, E. H., Judge, R. A., Crawford, L., Forsythe, E. L., Pusey, M. L., Sportiello, M., ... Borgstahl, G. E. (2001). Investigating the Effect of Impurities on Macromolecule Crystal Growth in Microgravity. Crystal Growth and Design, 1(2), 151-158. https://doi.org/10.1021/cg0055474

Investigating the Effect of Impurities on Macromolecule Crystal Growth in Microgravity. / Snell, Edward H.; Judge, Russell A.; Crawford, Lisa; Forsythe, Elizabeth L.; Pusey, Marc L.; Sportiello, Michael; Todd, Paul; Bellamy, Henry; Lovelace, Jeff; Cassanto, John M.; Borgstahl, Gloria E.

In: Crystal Growth and Design, Vol. 1, No. 2, 01.03.2001, p. 151-158.

Research output: Contribution to journalArticle

Snell, EH, Judge, RA, Crawford, L, Forsythe, EL, Pusey, ML, Sportiello, M, Todd, P, Bellamy, H, Lovelace, J, Cassanto, JM & Borgstahl, GE 2001, 'Investigating the Effect of Impurities on Macromolecule Crystal Growth in Microgravity', Crystal Growth and Design, vol. 1, no. 2, pp. 151-158. https://doi.org/10.1021/cg0055474
Snell EH, Judge RA, Crawford L, Forsythe EL, Pusey ML, Sportiello M et al. Investigating the Effect of Impurities on Macromolecule Crystal Growth in Microgravity. Crystal Growth and Design. 2001 Mar 1;1(2):151-158. https://doi.org/10.1021/cg0055474
Snell, Edward H. ; Judge, Russell A. ; Crawford, Lisa ; Forsythe, Elizabeth L. ; Pusey, Marc L. ; Sportiello, Michael ; Todd, Paul ; Bellamy, Henry ; Lovelace, Jeff ; Cassanto, John M. ; Borgstahl, Gloria E. / Investigating the Effect of Impurities on Macromolecule Crystal Growth in Microgravity. In: Crystal Growth and Design. 2001 ; Vol. 1, No. 2. pp. 151-158.
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