Intron position affects expression from the tpi promoter in rice

Kimberley C. Snowden, Wallace G. Buchhholz, Timothy C. Hall

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

A series of promoter-GUS fusion constructs containing a portion of the rice triosephosphate isomerase (tpi) promoter, the first tpi intron, and the gene encoding bacterial β-glucuronidase (GUS) were made. These constructs were electroporated into rice protoplasts and transient expression was monitored. Inclusion of the first intron from the rice tpi gene enhanced expression of the GUS gene from the tpi promoter when it was placed 5' of the GUS gene. When the tpi intron was placed in the 3'-untranslated region no enhancement of GUS gene expression was observed, indicating the importance of position in intron-mediated enhancement of gene expression.

Original languageEnglish (US)
Pages (from-to)689-692
Number of pages4
JournalPlant Molecular Biology
Volume31
Issue number3
DOIs
StatePublished - Jan 1 1996

Fingerprint

Triose-Phosphate Isomerase
triose-phosphate isomerase
Glucuronidase
Introns
introns
promoter regions
rice
Gene Expression
genes
Bacterial Genes
gene expression
Protoplasts
3' Untranslated Regions
3' untranslated regions
protoplasts
Genes
Oryza

Keywords

  • GUS
  • intron
  • rice
  • tpi
  • transient expression

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Genetics
  • Plant Science

Cite this

Intron position affects expression from the tpi promoter in rice. / Snowden, Kimberley C.; Buchhholz, Wallace G.; Hall, Timothy C.

In: Plant Molecular Biology, Vol. 31, No. 3, 01.01.1996, p. 689-692.

Research output: Contribution to journalArticle

Snowden, Kimberley C. ; Buchhholz, Wallace G. ; Hall, Timothy C. / Intron position affects expression from the tpi promoter in rice. In: Plant Molecular Biology. 1996 ; Vol. 31, No. 3. pp. 689-692.
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