Intracellular pH Effects in Lactic Acid Bacteria

Nancy L. Nannen, Robert W. Hutkins

Research output: Contribution to journalArticle

62 Citations (Scopus)

Abstract

The objectives of this research were to determine the effect of lactic acid and low pH on the intracellular pH in three species of lactic acid bacteria. A pH gradient (intracellular pH minus the extracellular pH) of .9 to 1.4 pH units was achieved by several strains of lactic acid bacteria, including Streptococcus thermophilus 19258 and 573, Lactococcus lactis ssp. lactis C2, and Lactococcus lactis ssp. cremoris HP during log phase of growth in various media. A noticeable decline of the pH gradient occurred at an internal pH of 5.5 to 6.0. In late stationary phase, the pH gradient was generally reduced to .5 pH units or less. In contrast, the aciduric Lactobacillus casei 685 maintained a large pH gradient (> 1.0 pH units) even when the medium pH was reduced to less than 4.0. Rapid growth of lactococci and streptococci in media containing excess lactose did not occur when the intracellular pH was reduced below a critical pH of 5.0 or at a neutral pH when proton-uncoupling agents were present.

Original languageEnglish (US)
Pages (from-to)741-746
Number of pages6
JournalJournal of Dairy Science
Volume74
Issue number3
DOIs
StatePublished - Jan 1 1991

Fingerprint

lactic acid bacteria
Lactic Acid
Bacteria
Proton-Motive Force
Lactococcus lactis
Uncoupling Agents
Lactococcus
Streptococcus thermophilus
Lactobacillus casei
Lactose
Growth
Streptococcus
Protons
lactic acid
protons
lactose
culture media

Keywords

  • CCCP
  • PEG
  • SMM
  • [H]polyethylene glycol
  • carbonyl cyanide m-chlorophenylhydrazone
  • extracellular pH
  • intracellular pH
  • intracellular pH
  • lactic acid
  • lactic acid bacteria
  • pH
  • pH
  • pH gradient (intracellular pH minus extracellular pH)
  • simulated milk medium
  • ΔpH

ASJC Scopus subject areas

  • Food Science
  • Animal Science and Zoology
  • Genetics

Cite this

Intracellular pH Effects in Lactic Acid Bacteria. / Nannen, Nancy L.; Hutkins, Robert W.

In: Journal of Dairy Science, Vol. 74, No. 3, 01.01.1991, p. 741-746.

Research output: Contribution to journalArticle

Nannen, Nancy L. ; Hutkins, Robert W. / Intracellular pH Effects in Lactic Acid Bacteria. In: Journal of Dairy Science. 1991 ; Vol. 74, No. 3. pp. 741-746.
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