Intestinal Mucosa Diamine Oxidase Activity Reflects Intestinal Involvement in Crohn's Disease

Jon S Thompson, D. A. Burnett, Rodney Smith Markin, W. P. Vaughan

Research output: Contribution to journalArticle

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Abstract

The activity of diamine oxidase (DAO), an enzyme found in the apical villous cells of the small intestine mucosa, should reflect the status of the intestinal mucosa. Our purpose was to determine whether DAO activity in the intestinal mucosa is diminished in patients with Crohn's disease and correlates with the severity of histological changes. Mucosal DAO activity was determined in 42 tissue specimens from patients with Crohn's enteritis (n = 15), Crohn's colitis (n = 9), and nicerative colitis (n = 11), and from patients with no intestinal disease (n = 7). DAO activity was estimated by the metabolism of [14C]putrescine. Histologic changes were graded on a scale of 0–4. Normal histology was graded as zero, mild edema, and inflammation in the lamina propria as one, crypt abscess formation and inflammation as two, more severe inflammation plus or minus granulomata as three, and most severe inflammation with active ulceration as four. Tissue DAO activity was significantly less in patients with ileitis (4.8 ± 3.6) compared with those with Crohn's colitis (15,0 ± 11.6), ulcerative colitis (16.8 ± 19.7), and normal intestine (17.6 ± 14.3 U/mg protein/h, p < 0.05). Intestinal DAO activity showed a positive correlation with the histologic scores. Recurrence of Crohn's disease developed postoperatively in two patients with low tissue DAO activity (1.4 and 2.9 U/mg protein/h). Intestinal DAO activity is diminished in patients with Crohn's ileitis and correlates with the severity of histologic changes. Tissue DAO activity might prove useful in predicting the risk of recurrence or anastomotic complications after resection for Crohn's disease.

Original languageEnglish (US)
Pages (from-to)756-760
Number of pages5
JournalThe American journal of gastroenterology
Volume83
Issue number7
DOIs
StatePublished - Jul 1988

Fingerprint

Amine Oxidase (Copper-Containing)
Intestinal Mucosa
Crohn Disease
Colitis
Ileitis
Inflammation
Mucous Membrane
Recurrence
Intestinal Diseases
Putrescine
Granuloma
Ulcerative Colitis
Abscess
Small Intestine
Intestines
Edema
Histology
Proteins

ASJC Scopus subject areas

  • Hepatology
  • Gastroenterology

Cite this

Intestinal Mucosa Diamine Oxidase Activity Reflects Intestinal Involvement in Crohn's Disease. / Thompson, Jon S; Burnett, D. A.; Markin, Rodney Smith; Vaughan, W. P.

In: The American journal of gastroenterology, Vol. 83, No. 7, 07.1988, p. 756-760.

Research output: Contribution to journalArticle

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