Intertumoral heterogeneity of receptor-tyrosine kinases expression in human melanoma cell lines with different metastatic capabilities

M. Gutman, Rakesh K Singh, R. Radinsky, M. Bar-Eli

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

Human melanocytes respond to several growth factors whose receptors have tyrosine kinase activity. Abnormalities in the expression of tyrosine kinase receptors may play an important role in the initiation and progression of melanoma. We therefore determined the steady-state mRNA expression of five tyrosine kinase receptors, epidermal growth factor receptor (EGF-R), c-met, nerve growth factor receptor (NGF-R), colony-stimulating factor receptor (CSF-R) and c-kit, in eleven human melanoma cell lines with different metastatic potentials in nude mice. All cell lines except for one nonmetastatic line established from a primary melanoma lost expression of c-kit. Expression of the other four tyrosine kinase receptors varied among the lines. The expression level of individual tyrosine kinase receptor did not correlate with the metastatic potential of the cells. These results suggest that metastatic human melanoma cell lines are heterogeneous for expression of tyrosine kinase receptors, with each cell type manifesting a distinct repertoire of receptor tyrosine kinases. The different profile of tyrosine kinase activities in different metastatic melanomas complicates its use for prognosis.

Original languageEnglish (US)
Pages (from-to)1759-1765
Number of pages7
JournalAnticancer Research
Volume14
Issue number5 A
StatePublished - Dec 1 1994

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Receptor Protein-Tyrosine Kinases
Melanoma
Cell Line
Protein-Tyrosine Kinases
Colony-Stimulating Factor Receptors
Nerve Growth Factor Receptor
Growth Factor Receptors
Melanocytes
Epidermal Growth Factor Receptor
Nude Mice
Messenger RNA

Keywords

  • Melanoma
  • Metastasis
  • Tyrosine kinase receptor

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Intertumoral heterogeneity of receptor-tyrosine kinases expression in human melanoma cell lines with different metastatic capabilities. / Gutman, M.; Singh, Rakesh K; Radinsky, R.; Bar-Eli, M.

In: Anticancer Research, Vol. 14, No. 5 A, 01.12.1994, p. 1759-1765.

Research output: Contribution to journalArticle

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AB - Human melanocytes respond to several growth factors whose receptors have tyrosine kinase activity. Abnormalities in the expression of tyrosine kinase receptors may play an important role in the initiation and progression of melanoma. We therefore determined the steady-state mRNA expression of five tyrosine kinase receptors, epidermal growth factor receptor (EGF-R), c-met, nerve growth factor receptor (NGF-R), colony-stimulating factor receptor (CSF-R) and c-kit, in eleven human melanoma cell lines with different metastatic potentials in nude mice. All cell lines except for one nonmetastatic line established from a primary melanoma lost expression of c-kit. Expression of the other four tyrosine kinase receptors varied among the lines. The expression level of individual tyrosine kinase receptor did not correlate with the metastatic potential of the cells. These results suggest that metastatic human melanoma cell lines are heterogeneous for expression of tyrosine kinase receptors, with each cell type manifesting a distinct repertoire of receptor tyrosine kinases. The different profile of tyrosine kinase activities in different metastatic melanomas complicates its use for prognosis.

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