Interrogating Protein Phosphatases with Chemical Activity Probes

Garrett R. Casey, Clifford I Stains

Research output: Contribution to journalReview article

5 Citations (Scopus)

Abstract

Protein phosphatases, while long overlooked, have recently become appreciated as drivers of both normal- and disease-associated signaling events. As a result, the spotlight is now turning torwards this enzyme family and efforts geared towards the development of modern chemical tools for studying these enzymes are well underway. This Minireview focuses on the evolution of chemical activity probes, both optical and covalent, for the study of protein phosphatases. Small-molecule probes, global monitoring of phosphatase activity through the use of covalent modifiers, and targeted fluorescence-based activity probes are discussed. We conclude with an overview of open questions in the field and highlight the potential impact of chemical tools for studying protein phosphatases.

Original languageEnglish (US)
Pages (from-to)7810-7824
Number of pages15
JournalChemistry - A European Journal
Volume24
Issue number31
DOIs
StatePublished - Jun 4 2018

Fingerprint

Phosphoprotein Phosphatases
Phosphatases
Catalyst activity
Proteins
Enzymes
Phosphoric Monoester Hydrolases
Fluorescence
Molecules
Monitoring

Keywords

  • biosensors
  • fluorescent probes
  • phosphatase
  • sensors
  • signal transduction

ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)
  • Organic Chemistry

Cite this

Interrogating Protein Phosphatases with Chemical Activity Probes. / Casey, Garrett R.; Stains, Clifford I.

In: Chemistry - A European Journal, Vol. 24, No. 31, 04.06.2018, p. 7810-7824.

Research output: Contribution to journalReview article

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