Interleukin 6 induction in the canine myocardium after cardiopulmonary bypass

William J. Dreyer, Sharon C. Phillips, Merry L Lindsey, Peggy Jackson, Neil E. Bowles, Lloyd H. Michael, Mark L. Entman

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Objective: Interleukin 6 is a proinflammatory cytokine with a plasma concentration that has been noted to increase in response to cardiopulmonary bypass. The source of interleukin 6 after cardiopulmonary bypass is unknown. This study examined the myocardium as a potential source of interleukin 6 in this context. Methods: Dogs underwent 90 minutes of hypothermic cardiopulmonary bypass with 60 minutes of cardioplegic arrest. After rewarming, they were reperfused with the chest open for either 3 (n = 4) or 6 (n = 4) hours, at the end of which myocardial samples were obtained. Four additional animals undergoing open thoracotomy without bypass served as time-matched controls. Northern blot analysis, reverse transcriptase-polymerase chain reaction, and in situ hybridization were used to examine the myocardium for the induction of interleukin 6 and intercellular adhesion molecule-1. Results: Northern blot analysis and reverse transcriptase-polymerase chain reaction demonstrated a marked increase in myocardial interleukin 6 messenger RNA in 3 of 4 dogs at 3 hours after bypass and 3 of 4 dogs at 6 hours after bypass, which was not present in sham-bypass control animals. Northern blots at 3 hours after cardiopulmonary bypass also demonstrated myocardial intercellular adhesion molecule-1 induction. In situ hybridization studies confirmed that cardiac myocytes were a principal source of interleukin 6 messenger RNA early after cardiopulmonary bypass. Northern blots of messenger RNA extracted from isolated neutrophils and mononuclear leukocytes obtained from blood samples before bypass, at the end of bypass, and 3 hours after bypass failed to demonstrate interleukin 6 induction. Conclusion: Despite protection with cold cardioplegic arrest, the myocardium was a significant source of interleukin 6 synthesis after cardiopulmonary bypass. Local production of interleukin 6 may play a pivotal role in postoperative myocardial function.

Original languageEnglish (US)
Pages (from-to)256-263
Number of pages8
JournalJournal of Thoracic and Cardiovascular Surgery
Volume120
Issue number2
DOIs
StatePublished - Jan 1 2000

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Cardiopulmonary Bypass
Canidae
Interleukin-6
Myocardium
Northern Blotting
Dogs
Intercellular Adhesion Molecule-1
Reverse Transcriptase Polymerase Chain Reaction
Messenger RNA
In Situ Hybridization
Mononuclear Leukocytes
Rewarming
Thoracotomy
Cardiac Myocytes
Neutrophils
Thorax
Cytokines

ASJC Scopus subject areas

  • Surgery
  • Pulmonary and Respiratory Medicine
  • Cardiology and Cardiovascular Medicine

Cite this

Dreyer, W. J., Phillips, S. C., Lindsey, M. L., Jackson, P., Bowles, N. E., Michael, L. H., & Entman, M. L. (2000). Interleukin 6 induction in the canine myocardium after cardiopulmonary bypass. Journal of Thoracic and Cardiovascular Surgery, 120(2), 256-263. https://doi.org/10.1067/mtc.2000.108168

Interleukin 6 induction in the canine myocardium after cardiopulmonary bypass. / Dreyer, William J.; Phillips, Sharon C.; Lindsey, Merry L; Jackson, Peggy; Bowles, Neil E.; Michael, Lloyd H.; Entman, Mark L.

In: Journal of Thoracic and Cardiovascular Surgery, Vol. 120, No. 2, 01.01.2000, p. 256-263.

Research output: Contribution to journalArticle

Dreyer, WJ, Phillips, SC, Lindsey, ML, Jackson, P, Bowles, NE, Michael, LH & Entman, ML 2000, 'Interleukin 6 induction in the canine myocardium after cardiopulmonary bypass', Journal of Thoracic and Cardiovascular Surgery, vol. 120, no. 2, pp. 256-263. https://doi.org/10.1067/mtc.2000.108168
Dreyer, William J. ; Phillips, Sharon C. ; Lindsey, Merry L ; Jackson, Peggy ; Bowles, Neil E. ; Michael, Lloyd H. ; Entman, Mark L. / Interleukin 6 induction in the canine myocardium after cardiopulmonary bypass. In: Journal of Thoracic and Cardiovascular Surgery. 2000 ; Vol. 120, No. 2. pp. 256-263.
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