Interleukin-4 secretion by the allograft fails to affect the allograft- specific interleukin-4 response in vitro

Joanna D. Davies, Regula Mueller, Susan Minson, Eric O'Connor, Troy Krahl, Nora Sarvetnick

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Background. The role of the cytokine, interleukin (IL)-4, in allograft rejection and protection is not clear. We have previously shown that IL-4 transgenically expressed in a pancreas allograft does not protect the allograft from rejection. Here, we analyze the effect of the transgenically expressed IL-4 on the cytokine profile of the allograft-specific immune response. Methods. C57BL/6SCID mice were infused with small numbers of spleen cells from C57BL/6 donors. The former received pancreas grafts from 1- to 2- day-old BALB/c donors which did or did not transgenically express IL-4 in the graft. Three weeks after the cell infusion, the spleens were removed and the splenocytes were restimulated in vitro with BALB/c APC, and third party BALB.K APC. IL-2 and IL-4 levels in the culture supernatants were measured. Results. The presence of a pancreatic allograft induced an increase in the levels of both IL-2 and IL-4 in culture supernatants from splenocytes of mice receiving grafts compared with mice not receiving grafts. The presence of IL- 4 transgenically expressed in the pancreas allograft had no effect on the in vitro cytokine profile. Conclusions. From these results we conclude that the failure of transgenically expressed IL-4 to protect the allograft was not associated with up-regulation of a graft antigen-specific IL-4 response.

Original languageEnglish (US)
Pages (from-to)1583-1589
Number of pages7
JournalTransplantation
Volume67
Issue number12
DOIs
StatePublished - Jun 27 1999

Fingerprint

Interleukin-4
Allografts
Transplants
Pancreas
Cytokines
Interleukin-2
Spleen
In Vitro Techniques
Inbred C57BL Mouse
Up-Regulation
Cell Count
Antigens

ASJC Scopus subject areas

  • Transplantation

Cite this

Interleukin-4 secretion by the allograft fails to affect the allograft- specific interleukin-4 response in vitro. / Davies, Joanna D.; Mueller, Regula; Minson, Susan; O'Connor, Eric; Krahl, Troy; Sarvetnick, Nora.

In: Transplantation, Vol. 67, No. 12, 27.06.1999, p. 1583-1589.

Research output: Contribution to journalArticle

Davies, Joanna D. ; Mueller, Regula ; Minson, Susan ; O'Connor, Eric ; Krahl, Troy ; Sarvetnick, Nora. / Interleukin-4 secretion by the allograft fails to affect the allograft- specific interleukin-4 response in vitro. In: Transplantation. 1999 ; Vol. 67, No. 12. pp. 1583-1589.
@article{82be7ac53c654ca282ff4b974bcd5b74,
title = "Interleukin-4 secretion by the allograft fails to affect the allograft- specific interleukin-4 response in vitro",
abstract = "Background. The role of the cytokine, interleukin (IL)-4, in allograft rejection and protection is not clear. We have previously shown that IL-4 transgenically expressed in a pancreas allograft does not protect the allograft from rejection. Here, we analyze the effect of the transgenically expressed IL-4 on the cytokine profile of the allograft-specific immune response. Methods. C57BL/6SCID mice were infused with small numbers of spleen cells from C57BL/6 donors. The former received pancreas grafts from 1- to 2- day-old BALB/c donors which did or did not transgenically express IL-4 in the graft. Three weeks after the cell infusion, the spleens were removed and the splenocytes were restimulated in vitro with BALB/c APC, and third party BALB.K APC. IL-2 and IL-4 levels in the culture supernatants were measured. Results. The presence of a pancreatic allograft induced an increase in the levels of both IL-2 and IL-4 in culture supernatants from splenocytes of mice receiving grafts compared with mice not receiving grafts. The presence of IL- 4 transgenically expressed in the pancreas allograft had no effect on the in vitro cytokine profile. Conclusions. From these results we conclude that the failure of transgenically expressed IL-4 to protect the allograft was not associated with up-regulation of a graft antigen-specific IL-4 response.",
author = "Davies, {Joanna D.} and Regula Mueller and Susan Minson and Eric O'Connor and Troy Krahl and Nora Sarvetnick",
year = "1999",
month = "6",
day = "27",
doi = "10.1097/00007890-199906270-00013",
language = "English (US)",
volume = "67",
pages = "1583--1589",
journal = "Transplantation",
issn = "0041-1337",
publisher = "Lippincott Williams and Wilkins",
number = "12",

}

TY - JOUR

T1 - Interleukin-4 secretion by the allograft fails to affect the allograft- specific interleukin-4 response in vitro

AU - Davies, Joanna D.

AU - Mueller, Regula

AU - Minson, Susan

AU - O'Connor, Eric

AU - Krahl, Troy

AU - Sarvetnick, Nora

PY - 1999/6/27

Y1 - 1999/6/27

N2 - Background. The role of the cytokine, interleukin (IL)-4, in allograft rejection and protection is not clear. We have previously shown that IL-4 transgenically expressed in a pancreas allograft does not protect the allograft from rejection. Here, we analyze the effect of the transgenically expressed IL-4 on the cytokine profile of the allograft-specific immune response. Methods. C57BL/6SCID mice were infused with small numbers of spleen cells from C57BL/6 donors. The former received pancreas grafts from 1- to 2- day-old BALB/c donors which did or did not transgenically express IL-4 in the graft. Three weeks after the cell infusion, the spleens were removed and the splenocytes were restimulated in vitro with BALB/c APC, and third party BALB.K APC. IL-2 and IL-4 levels in the culture supernatants were measured. Results. The presence of a pancreatic allograft induced an increase in the levels of both IL-2 and IL-4 in culture supernatants from splenocytes of mice receiving grafts compared with mice not receiving grafts. The presence of IL- 4 transgenically expressed in the pancreas allograft had no effect on the in vitro cytokine profile. Conclusions. From these results we conclude that the failure of transgenically expressed IL-4 to protect the allograft was not associated with up-regulation of a graft antigen-specific IL-4 response.

AB - Background. The role of the cytokine, interleukin (IL)-4, in allograft rejection and protection is not clear. We have previously shown that IL-4 transgenically expressed in a pancreas allograft does not protect the allograft from rejection. Here, we analyze the effect of the transgenically expressed IL-4 on the cytokine profile of the allograft-specific immune response. Methods. C57BL/6SCID mice were infused with small numbers of spleen cells from C57BL/6 donors. The former received pancreas grafts from 1- to 2- day-old BALB/c donors which did or did not transgenically express IL-4 in the graft. Three weeks after the cell infusion, the spleens were removed and the splenocytes were restimulated in vitro with BALB/c APC, and third party BALB.K APC. IL-2 and IL-4 levels in the culture supernatants were measured. Results. The presence of a pancreatic allograft induced an increase in the levels of both IL-2 and IL-4 in culture supernatants from splenocytes of mice receiving grafts compared with mice not receiving grafts. The presence of IL- 4 transgenically expressed in the pancreas allograft had no effect on the in vitro cytokine profile. Conclusions. From these results we conclude that the failure of transgenically expressed IL-4 to protect the allograft was not associated with up-regulation of a graft antigen-specific IL-4 response.

UR - http://www.scopus.com/inward/record.url?scp=0032976418&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032976418&partnerID=8YFLogxK

U2 - 10.1097/00007890-199906270-00013

DO - 10.1097/00007890-199906270-00013

M3 - Article

C2 - 10401766

AN - SCOPUS:0032976418

VL - 67

SP - 1583

EP - 1589

JO - Transplantation

JF - Transplantation

SN - 0041-1337

IS - 12

ER -