Interleukin-21 enhances the antibody avidity elicited by DNA prime and MVA boost vaccine

Wenjin Fan, Yanmin Wan, Qingsheng Li

Research output: Contribution to journalArticle

Abstract

Enhancement of the magnitude or affinity of protective antibodies (Abs) induced by vaccine adjuvant is highly desirable to prevent challenging pathogens such as HIV-1. IL-21 plays a crucial role in germinal center reactions during humoral immune responses. However, the effect of IL-21 as a vaccine adjuvant on the quantity and quality of antigen-specific Abs elicited by DNA prime and MVA boost vaccine, a commonly used vaccine strategy, remains unknown. To close this knowledge gap, female adult B6N mice were primed with DNA vaccine twice (days 0, 14, 100 µg, I.M.) and boosted with MVA vaccine (day 28, 2 × 107 pfu, I.M.) with or without an IL-21 DNA adjuvant (days 3, 17, 31, 40 µg, I.M.), in which HIV-1 gag was expressed as a model antigen. With the addition of an IL-21 adjuvant, we found significantly increased avidity of antigen-specific Abs at multiple time points in a longitudinal follow up. Collectively, our results suggest that an IL-21 immune adjuvant can significantly increase Ab quality induced by heterologous DNA-MVA prime-boost vaccine strategy.

Original languageEnglish (US)
Article number154814
JournalCytokine
Volume125
DOIs
StatePublished - Jan 2020

Fingerprint

Antibody Affinity
Vaccines
Antibodies
DNA
Antigens
HIV-1
DNA Vaccines
Germinal Center
Pathogens
Humoral Immunity
MVA vaccine
interleukin-21

Keywords

  • Antibody avidity
  • DNA vaccine
  • IL-21
  • MVA

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Biochemistry
  • Hematology
  • Molecular Biology

Cite this

Interleukin-21 enhances the antibody avidity elicited by DNA prime and MVA boost vaccine. / Fan, Wenjin; Wan, Yanmin; Li, Qingsheng.

In: Cytokine, Vol. 125, 154814, 01.2020.

Research output: Contribution to journalArticle

@article{a4b57970af8c4d99b5b3373c0acf9dcf,
title = "Interleukin-21 enhances the antibody avidity elicited by DNA prime and MVA boost vaccine",
abstract = "Enhancement of the magnitude or affinity of protective antibodies (Abs) induced by vaccine adjuvant is highly desirable to prevent challenging pathogens such as HIV-1. IL-21 plays a crucial role in germinal center reactions during humoral immune responses. However, the effect of IL-21 as a vaccine adjuvant on the quantity and quality of antigen-specific Abs elicited by DNA prime and MVA boost vaccine, a commonly used vaccine strategy, remains unknown. To close this knowledge gap, female adult B6N mice were primed with DNA vaccine twice (days 0, 14, 100 µg, I.M.) and boosted with MVA vaccine (day 28, 2 × 107 pfu, I.M.) with or without an IL-21 DNA adjuvant (days 3, 17, 31, 40 µg, I.M.), in which HIV-1 gag was expressed as a model antigen. With the addition of an IL-21 adjuvant, we found significantly increased avidity of antigen-specific Abs at multiple time points in a longitudinal follow up. Collectively, our results suggest that an IL-21 immune adjuvant can significantly increase Ab quality induced by heterologous DNA-MVA prime-boost vaccine strategy.",
keywords = "Antibody avidity, DNA vaccine, IL-21, MVA",
author = "Wenjin Fan and Yanmin Wan and Qingsheng Li",
year = "2020",
month = "1",
doi = "10.1016/j.cyto.2019.154814",
language = "English (US)",
volume = "125",
journal = "Cytokine",
issn = "1043-4666",
publisher = "Academic Press Inc.",

}

TY - JOUR

T1 - Interleukin-21 enhances the antibody avidity elicited by DNA prime and MVA boost vaccine

AU - Fan, Wenjin

AU - Wan, Yanmin

AU - Li, Qingsheng

PY - 2020/1

Y1 - 2020/1

N2 - Enhancement of the magnitude or affinity of protective antibodies (Abs) induced by vaccine adjuvant is highly desirable to prevent challenging pathogens such as HIV-1. IL-21 plays a crucial role in germinal center reactions during humoral immune responses. However, the effect of IL-21 as a vaccine adjuvant on the quantity and quality of antigen-specific Abs elicited by DNA prime and MVA boost vaccine, a commonly used vaccine strategy, remains unknown. To close this knowledge gap, female adult B6N mice were primed with DNA vaccine twice (days 0, 14, 100 µg, I.M.) and boosted with MVA vaccine (day 28, 2 × 107 pfu, I.M.) with or without an IL-21 DNA adjuvant (days 3, 17, 31, 40 µg, I.M.), in which HIV-1 gag was expressed as a model antigen. With the addition of an IL-21 adjuvant, we found significantly increased avidity of antigen-specific Abs at multiple time points in a longitudinal follow up. Collectively, our results suggest that an IL-21 immune adjuvant can significantly increase Ab quality induced by heterologous DNA-MVA prime-boost vaccine strategy.

AB - Enhancement of the magnitude or affinity of protective antibodies (Abs) induced by vaccine adjuvant is highly desirable to prevent challenging pathogens such as HIV-1. IL-21 plays a crucial role in germinal center reactions during humoral immune responses. However, the effect of IL-21 as a vaccine adjuvant on the quantity and quality of antigen-specific Abs elicited by DNA prime and MVA boost vaccine, a commonly used vaccine strategy, remains unknown. To close this knowledge gap, female adult B6N mice were primed with DNA vaccine twice (days 0, 14, 100 µg, I.M.) and boosted with MVA vaccine (day 28, 2 × 107 pfu, I.M.) with or without an IL-21 DNA adjuvant (days 3, 17, 31, 40 µg, I.M.), in which HIV-1 gag was expressed as a model antigen. With the addition of an IL-21 adjuvant, we found significantly increased avidity of antigen-specific Abs at multiple time points in a longitudinal follow up. Collectively, our results suggest that an IL-21 immune adjuvant can significantly increase Ab quality induced by heterologous DNA-MVA prime-boost vaccine strategy.

KW - Antibody avidity

KW - DNA vaccine

KW - IL-21

KW - MVA

UR - http://www.scopus.com/inward/record.url?scp=85070969370&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85070969370&partnerID=8YFLogxK

U2 - 10.1016/j.cyto.2019.154814

DO - 10.1016/j.cyto.2019.154814

M3 - Article

C2 - 31450102

AN - SCOPUS:85070969370

VL - 125

JO - Cytokine

JF - Cytokine

SN - 1043-4666

M1 - 154814

ER -