Interleukin-2 receptor-γ-dependent endocytosis depends on biotin in jurkat cells

Rocio Rodriguez-Melendez, Gabriela Camporeale, Jacob B. Griffin, Janos Zempleni

Research output: Contribution to journalArticle

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Abstract

Biotin has been credited with having beneficial effects on immune function despite observations that biotin supplementation causes decreased secretion of interleukin-2. Here this paradox was addressed by determining whether receptor-dependent internalization of interleukin-2 by immune cells depends on biotin. Theoretically, this would be consistent with both decreased net secretion of interleukin-2 by biotin-supplemented cells (causing increased endocytosis) and beneficial effects of biotin on immune function (causing increased receptor signaling). Jurkat cells were cultured in biotin-defined media (25, 250, or 10,000 pM). Secretion of interleukin-2 correlated negatively with biotin supply, but transcriptional activity of the interleukin-2 gene correlated positively with biotin supply, suggesting that decreased secretion of interleukin-2 by biotin-supplemented cells was not caused by decreased gene expression. Expression of the interleukin-2 receptor-γ gene was greater at 10,000 pM than 25 pM biotin, mediating increased endocytosis of interleukin-2 in biotin-supplemented medium. Inhibition of endocytosis by genistein and overexpression of interleukin-2 receptor-γ abolished the effect of biotin. These findings suggest that endocytosis of interleukin-2 depends on biotin.

Original languageEnglish (US)
Pages (from-to)C415-C421
JournalAmerican Journal of Physiology - Cell Physiology
Volume284
Issue number2 53-2
Publication statusPublished - Feb 1 2003

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Keywords

  • Cytokines
  • Gene expression
  • Propionyl-CoA carboxylase

ASJC Scopus subject areas

  • Physiology
  • Cell Biology

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