Interleukin-2-induced chemotaxis of human T-lymphocytes

Richard A. Robbins, Lynell Warren Klassen, Julie Rasmussen, M. E M Clayton, Wesley D. Russ

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Interleukin-2 (IL-2), a growth factor for T-lymphocytes, has been postulated to cause the accumulation of T-lymphocytes at sites of inflammation by inducing proliferation of these cells. We hypothesized that IL-2 might also serve to attract T-lymphocytes to inflammatory sites. To test this hypothesis, human T-lymphocytes were purified from the peripheral blood of normal volunteers by rosetting with neuraminidase-treated sheep red blood cells and tested for chemotactic activity by using a blind-well chamber technique. Purified IL-2 caused a greater than 20-fold attraction of T-lymphocytes compared with medium alone (P < 0.001). This attraction was shown to be chemotactic rather than chemokinetic by checkerboard analysis. The T-lymphocyte chemotaxis could be completely inhibited by adsorption of the IL-2 with an IL-2-dependent cell line, and could be neutralized by monoclonal anti-IL-2 antibody. Further specificity of IL-2-directed chemotaxis was demonstrated by using species-specific IL-2. Mouse IL-2 was ineffective at promoting human T-lymphocyte chemotaxis. These data suggest that IL-2 may be responsible for the localized accumulation of T-lymphocytes both by causing attraction of these cells and by modulating their proliferation.

Original languageEnglish (US)
Pages (from-to)332-339
Number of pages8
JournalThe Journal of Laboratory and Clinical Medicine
Volume108
Issue number4
StatePublished - Jan 1 1986

Fingerprint

T-cells
Chemotaxis
Interleukin-2
T-Lymphocytes
Blood
Cells
Neuraminidase
Adsorption
Sheep
Intercellular Signaling Peptides and Proteins
Healthy Volunteers
Erythrocytes
Cell Proliferation
Inflammation
Cell Line

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Robbins, R. A., Klassen, L. W., Rasmussen, J., Clayton, M. E. M., & Russ, W. D. (1986). Interleukin-2-induced chemotaxis of human T-lymphocytes. The Journal of Laboratory and Clinical Medicine, 108(4), 332-339.

Interleukin-2-induced chemotaxis of human T-lymphocytes. / Robbins, Richard A.; Klassen, Lynell Warren; Rasmussen, Julie; Clayton, M. E M; Russ, Wesley D.

In: The Journal of Laboratory and Clinical Medicine, Vol. 108, No. 4, 01.01.1986, p. 332-339.

Research output: Contribution to journalArticle

Robbins, RA, Klassen, LW, Rasmussen, J, Clayton, MEM & Russ, WD 1986, 'Interleukin-2-induced chemotaxis of human T-lymphocytes', The Journal of Laboratory and Clinical Medicine, vol. 108, no. 4, pp. 332-339.
Robbins RA, Klassen LW, Rasmussen J, Clayton MEM, Russ WD. Interleukin-2-induced chemotaxis of human T-lymphocytes. The Journal of Laboratory and Clinical Medicine. 1986 Jan 1;108(4):332-339.
Robbins, Richard A. ; Klassen, Lynell Warren ; Rasmussen, Julie ; Clayton, M. E M ; Russ, Wesley D. / Interleukin-2-induced chemotaxis of human T-lymphocytes. In: The Journal of Laboratory and Clinical Medicine. 1986 ; Vol. 108, No. 4. pp. 332-339.
@article{b9be6da8137a4c508e0df78705e43ea9,
title = "Interleukin-2-induced chemotaxis of human T-lymphocytes",
abstract = "Interleukin-2 (IL-2), a growth factor for T-lymphocytes, has been postulated to cause the accumulation of T-lymphocytes at sites of inflammation by inducing proliferation of these cells. We hypothesized that IL-2 might also serve to attract T-lymphocytes to inflammatory sites. To test this hypothesis, human T-lymphocytes were purified from the peripheral blood of normal volunteers by rosetting with neuraminidase-treated sheep red blood cells and tested for chemotactic activity by using a blind-well chamber technique. Purified IL-2 caused a greater than 20-fold attraction of T-lymphocytes compared with medium alone (P < 0.001). This attraction was shown to be chemotactic rather than chemokinetic by checkerboard analysis. The T-lymphocyte chemotaxis could be completely inhibited by adsorption of the IL-2 with an IL-2-dependent cell line, and could be neutralized by monoclonal anti-IL-2 antibody. Further specificity of IL-2-directed chemotaxis was demonstrated by using species-specific IL-2. Mouse IL-2 was ineffective at promoting human T-lymphocyte chemotaxis. These data suggest that IL-2 may be responsible for the localized accumulation of T-lymphocytes both by causing attraction of these cells and by modulating their proliferation.",
author = "Robbins, {Richard A.} and Klassen, {Lynell Warren} and Julie Rasmussen and Clayton, {M. E M} and Russ, {Wesley D.}",
year = "1986",
month = "1",
day = "1",
language = "English (US)",
volume = "108",
pages = "332--339",
journal = "Translational Research",
issn = "1931-5244",
publisher = "Mosby Inc.",
number = "4",

}

TY - JOUR

T1 - Interleukin-2-induced chemotaxis of human T-lymphocytes

AU - Robbins, Richard A.

AU - Klassen, Lynell Warren

AU - Rasmussen, Julie

AU - Clayton, M. E M

AU - Russ, Wesley D.

PY - 1986/1/1

Y1 - 1986/1/1

N2 - Interleukin-2 (IL-2), a growth factor for T-lymphocytes, has been postulated to cause the accumulation of T-lymphocytes at sites of inflammation by inducing proliferation of these cells. We hypothesized that IL-2 might also serve to attract T-lymphocytes to inflammatory sites. To test this hypothesis, human T-lymphocytes were purified from the peripheral blood of normal volunteers by rosetting with neuraminidase-treated sheep red blood cells and tested for chemotactic activity by using a blind-well chamber technique. Purified IL-2 caused a greater than 20-fold attraction of T-lymphocytes compared with medium alone (P < 0.001). This attraction was shown to be chemotactic rather than chemokinetic by checkerboard analysis. The T-lymphocyte chemotaxis could be completely inhibited by adsorption of the IL-2 with an IL-2-dependent cell line, and could be neutralized by monoclonal anti-IL-2 antibody. Further specificity of IL-2-directed chemotaxis was demonstrated by using species-specific IL-2. Mouse IL-2 was ineffective at promoting human T-lymphocyte chemotaxis. These data suggest that IL-2 may be responsible for the localized accumulation of T-lymphocytes both by causing attraction of these cells and by modulating their proliferation.

AB - Interleukin-2 (IL-2), a growth factor for T-lymphocytes, has been postulated to cause the accumulation of T-lymphocytes at sites of inflammation by inducing proliferation of these cells. We hypothesized that IL-2 might also serve to attract T-lymphocytes to inflammatory sites. To test this hypothesis, human T-lymphocytes were purified from the peripheral blood of normal volunteers by rosetting with neuraminidase-treated sheep red blood cells and tested for chemotactic activity by using a blind-well chamber technique. Purified IL-2 caused a greater than 20-fold attraction of T-lymphocytes compared with medium alone (P < 0.001). This attraction was shown to be chemotactic rather than chemokinetic by checkerboard analysis. The T-lymphocyte chemotaxis could be completely inhibited by adsorption of the IL-2 with an IL-2-dependent cell line, and could be neutralized by monoclonal anti-IL-2 antibody. Further specificity of IL-2-directed chemotaxis was demonstrated by using species-specific IL-2. Mouse IL-2 was ineffective at promoting human T-lymphocyte chemotaxis. These data suggest that IL-2 may be responsible for the localized accumulation of T-lymphocytes both by causing attraction of these cells and by modulating their proliferation.

UR - http://www.scopus.com/inward/record.url?scp=46149130758&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=46149130758&partnerID=8YFLogxK

M3 - Article

VL - 108

SP - 332

EP - 339

JO - Translational Research

JF - Translational Research

SN - 1931-5244

IS - 4

ER -