Interleukin-2-induced chemotaxis of human T-lymphocytes

R. A. Robbins, L. Klassen, J. Rasmussen, M. E. Clayton, W. D. Russ

Research output: Contribution to journalArticle

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Abstract

Interleukin-2 (IL-2), a growth factor for T-lymphocytes, has been postulated to cause the accumulation of T-lymphocytes at sites of inflammation by inducing proliferation of these cells. We hypothesized that IL-2 might also serve to attract T-lymphocytes to inflammatory sites. To test this hypothesis, human T-lymphocytes were purified from the peripheral blood of normal volunteers by rosetting with neuraminidase-treated sheep red blood cells and tested for chemotactic activity by using a blind-well chamber technique. Purified IL-2 caused a greater than 20-fold attraction of T-lymphocytes compared with medium alone (P < 0.001). This attraction was shown to be chemotactic rather than chemokinetic by checkerboard analysis. The T-lymphocyte chemotaxis could be completely inhibited by adsorption of the IL-2 with an IL-2-dependent cell line, and could be neutralized by monoclonal anti-IL-2 antibody. Further specificity of IL-2-directed chemotaxis was demonstrated by using species-specific IL-2. Mouse IL-2 was ineffective at promoting human T-lymphocyte chemotaxis. These data suggest that IL-2 may be responsible for the localized accumulation of T-lymphocytes both by causing attraction of these cells and by modulating their proliferation.

Original languageEnglish (US)
Pages (from-to)340-345
Number of pages6
JournalJournal of Laboratory and Clinical Medicine
Volume108
Issue number4
StatePublished - Dec 19 1986

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T-cells
Chemotaxis
Interleukin-2
T-Lymphocytes
Blood
Cells
Neuraminidase
Adsorption
Sheep
Intercellular Signaling Peptides and Proteins
Healthy Volunteers
Erythrocytes
Cell Proliferation
Inflammation
Cell Line

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Robbins, R. A., Klassen, L., Rasmussen, J., Clayton, M. E., & Russ, W. D. (1986). Interleukin-2-induced chemotaxis of human T-lymphocytes. Journal of Laboratory and Clinical Medicine, 108(4), 340-345.

Interleukin-2-induced chemotaxis of human T-lymphocytes. / Robbins, R. A.; Klassen, L.; Rasmussen, J.; Clayton, M. E.; Russ, W. D.

In: Journal of Laboratory and Clinical Medicine, Vol. 108, No. 4, 19.12.1986, p. 340-345.

Research output: Contribution to journalArticle

Robbins, RA, Klassen, L, Rasmussen, J, Clayton, ME & Russ, WD 1986, 'Interleukin-2-induced chemotaxis of human T-lymphocytes', Journal of Laboratory and Clinical Medicine, vol. 108, no. 4, pp. 340-345.
Robbins RA, Klassen L, Rasmussen J, Clayton ME, Russ WD. Interleukin-2-induced chemotaxis of human T-lymphocytes. Journal of Laboratory and Clinical Medicine. 1986 Dec 19;108(4):340-345.
Robbins, R. A. ; Klassen, L. ; Rasmussen, J. ; Clayton, M. E. ; Russ, W. D. / Interleukin-2-induced chemotaxis of human T-lymphocytes. In: Journal of Laboratory and Clinical Medicine. 1986 ; Vol. 108, No. 4. pp. 340-345.
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