Interleukin 2 exerts autocrine stimulation on murine T-cell leukaemia growth

C. I. Waldner, C. Mongini, E. Alvarez, M. Sánchez Lockhart, M. J. Gravisaco, S. E. Hajos

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

As it has been suggested that an autocrine mechanism may control tumour cell growth, in this work cells from a spontaneous murine T lymphocyte leukaemia (LB) expressing the interleukin-2 receptor (IL-2R) (CD25) were evaluated in vitro for IL-2-mediated autocrine growth. Cells grew readily in culture and proliferation was enhanced by the addition of recombinant IL-2 but inhibited by monoclonal antibodies against either IL-2 or IL-2 receptor, in the absence of exogenous IL-2. Cyclosporin A also inhibited LB cell growth. However, when exogenous IL-2 was added together with cyclosporin A, cell proliferation proved similar to controls. Using reverse transcription polymerase chain reaction (PCR), mRNA for IL-2 was found to be present in tumour cells. Our findings support the hypothesis that LB tumour cell proliferation is mediated by an autocrine pathway involving endogenous IL-2 generation, despite the fact that these cells are not dependent on exogenous IL-2 to grow in culture.

Original languageEnglish (US)
Pages (from-to)946-950
Number of pages5
JournalBritish journal of cancer
Volume75
Issue number7
DOIs
StatePublished - Jan 1 1997

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Keywords

  • Autocrine growth
  • Interleukin 2
  • Murine leukaemia

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Waldner, C. I., Mongini, C., Alvarez, E., Sánchez Lockhart, M., Gravisaco, M. J., & Hajos, S. E. (1997). Interleukin 2 exerts autocrine stimulation on murine T-cell leukaemia growth. British journal of cancer, 75(7), 946-950. https://doi.org/10.1038/bjc.1997.165