Interleukin 2 exerts autocrine stimulation on murine T-cell leukaemia growth

C. I. Waldner, C. Mongini, E. Alvarez, Mariano Sanchez-Lockhart, M. J. Gravisaco, S. E. Hajos

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

As it has been suggested that an autocrine mechanism may control tumour cell growth, in this work cells from a spontaneous murine T lymphocyte leukaemia (LB) expressing the interleukin-2 receptor (IL-2R) (CD25) were evaluated in vitro for IL-2-mediated autocrine growth. Cells grew readily in culture and proliferation was enhanced by the addition of recombinant IL-2 but inhibited by monoclonal antibodies against either IL-2 or IL-2 receptor, in the absence of exogenous IL-2. Cyclosporin A also inhibited LB cell growth. However, when exogenous IL-2 was added together with cyclosporin A, cell proliferation proved similar to controls. Using reverse transcription polymerase chain reaction (PCR), mRNA for IL-2 was found to be present in tumour cells. Our findings support the hypothesis that LB tumour cell proliferation is mediated by an autocrine pathway involving endogenous IL-2 generation, despite the fact that these cells are not dependent on exogenous IL-2 to grow in culture.

Original languageEnglish (US)
Pages (from-to)946-950
Number of pages5
JournalBritish journal of cancer
Volume75
Issue number7
DOIs
StatePublished - Jan 1 1997

Fingerprint

T-Cell Leukemia
Interleukin-2
Growth
Interleukin-2 Receptors
Cyclosporine
Cell Proliferation
Neoplasms
Reverse Transcription
Leukemia
Monoclonal Antibodies
T-Lymphocytes
Polymerase Chain Reaction
Messenger RNA

Keywords

  • Autocrine growth
  • Interleukin 2
  • Murine leukaemia

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Interleukin 2 exerts autocrine stimulation on murine T-cell leukaemia growth. / Waldner, C. I.; Mongini, C.; Alvarez, E.; Sanchez-Lockhart, Mariano; Gravisaco, M. J.; Hajos, S. E.

In: British journal of cancer, Vol. 75, No. 7, 01.01.1997, p. 946-950.

Research output: Contribution to journalArticle

Waldner, C. I. ; Mongini, C. ; Alvarez, E. ; Sanchez-Lockhart, Mariano ; Gravisaco, M. J. ; Hajos, S. E. / Interleukin 2 exerts autocrine stimulation on murine T-cell leukaemia growth. In: British journal of cancer. 1997 ; Vol. 75, No. 7. pp. 946-950.
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