Interferons α and β down-regulate the expression of basic fibroblast growth factor in human carcinomas

Rakesh K Singh, Mordechai Gutman, Corazon D. Bucana, Ricardo Sanchez, Norma Llansa, Isaiah J. Fidler

Research output: Contribution to journalArticle

403 Citations (Scopus)

Abstract

We investigated the influence of interferons α, β, and γ (IFN-α, -β, and -γ) on the production of basic fibroblast growth factor (bFGF) by human renal carcinoma cells. The human renal carcinoma cell metastatic line SN12PM6 was established in culture from a lung metastasis and SN12PM6-resistant cells were selected in vitro for resistance to the antiproliferative effects of IFN-α or IFN-β. IFN-α and IFN-β, but not IFN-γ, down-regulated the expression of bFGF at the mRNA and protein levels by a mechanism independent of their antiproliferative effects. Downregulation of bFGF required a long exposure (>4 days) of cells to low concentrations (> 10 units/ml) of IFN-α or IFN-β. The withdrawal of IFN-α or IFN-β from the medium permitted SN12PM6-resistant cells to resume production of bFGF. The incubation of human bladder, prostate, colon, and breast carcinoma cells with noncytostatic concentrations of IFN-α or IFN-β also produced down-regulation of bFGF production.

Original languageEnglish (US)
Pages (from-to)4562-4566
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume92
Issue number10
DOIs
StatePublished - May 9 1995

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Fibroblast Growth Factor 2
Interferons
Down-Regulation
Carcinoma
Renal Cell Carcinoma
Prostate
Colon
Urinary Bladder
Breast Neoplasms
Neoplasm Metastasis
Kidney
Cell Line
Lung
Messenger RNA
Proteins

Keywords

  • angiogenesis

ASJC Scopus subject areas

  • General

Cite this

Interferons α and β down-regulate the expression of basic fibroblast growth factor in human carcinomas. / Singh, Rakesh K; Gutman, Mordechai; Bucana, Corazon D.; Sanchez, Ricardo; Llansa, Norma; Fidler, Isaiah J.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 92, No. 10, 09.05.1995, p. 4562-4566.

Research output: Contribution to journalArticle

Singh, Rakesh K ; Gutman, Mordechai ; Bucana, Corazon D. ; Sanchez, Ricardo ; Llansa, Norma ; Fidler, Isaiah J. / Interferons α and β down-regulate the expression of basic fibroblast growth factor in human carcinomas. In: Proceedings of the National Academy of Sciences of the United States of America. 1995 ; Vol. 92, No. 10. pp. 4562-4566.
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