Interferon regulatory factor 7 is associated with Epstein-Barr virus-transformed central nervous system lymphoma and has oncogenic properties

Luwen Zhang, Jun Zhang, Que Lambert, Channing J. Der, Luis Del Valle, Judith Miklossy, Kamel Khalili, You Zhou, Joseph S. Pagano

Research output: Contribution to journalArticle

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Abstract

Interferon regulatory factor 7 (IRF-7) is implicated in the regulation of Epstein-Barr virus (EBV) latency. EBV transforms primary B cells, and the major EBV oncoprotein, latent membrane protein 1 (LMP-1), is required for the process. LMP-1 both induces the expression of IRF-7 and activates the IRF-7 protein by phosphorylation and nuclear translocation. Here we report that the expression of IRF-7 is increased in EBV-imraortalized B lymphocytes compared with that in primary B cells. IRF-7 was phosphorylated and predominantly localized in the nucleus in the immortalized cells. The expression of IRF-7 was detected in 19 of 27 specimens of primary lymphomas of the human central nervous system by immunohistochemical analysis. The association between LMP-1 and IRF-7 was statistically highly significant for these specimens. An appreciable amount of the IRF-7 expressed in lymphoma cells was localized in the nucleus. Furthermore, IRF-7 promoted the anchorage-independent growth of NIH 3T3 cells. LMP-1 and IRF-7 showed additive effects on the growth transformation of NIH 3T3 cells. IRF-7-expressing NIH 3T3 cells formed tumors in athymic mice. Thus, IRF-7 has oncogenic properties and, along with LMP-1, may mediate or potentiate the EBV transformation process in the pathogenesis of EBV-associated lymphomas.

Original languageEnglish (US)
Pages (from-to)12987-12995
Number of pages9
JournalJournal of virology
Volume78
Issue number23
DOIs
StatePublished - Dec 1 2004

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Interferon Regulatory Factor-7
Human herpesvirus 4
lymphoma
Human Herpesvirus 4
central nervous system
Lymphoma
Central Nervous System
membrane proteins
NIH 3T3 Cells
Membrane Proteins
B-lymphocytes
B-Lymphocytes
interferon regulatory factor-7
cells
Virus Latency
Oncogene Proteins
cell nucleus
Growth
Nuclear Proteins

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

Cite this

Interferon regulatory factor 7 is associated with Epstein-Barr virus-transformed central nervous system lymphoma and has oncogenic properties. / Zhang, Luwen; Zhang, Jun; Lambert, Que; Der, Channing J.; Del Valle, Luis; Miklossy, Judith; Khalili, Kamel; Zhou, You; Pagano, Joseph S.

In: Journal of virology, Vol. 78, No. 23, 01.12.2004, p. 12987-12995.

Research output: Contribution to journalArticle

Zhang, Luwen ; Zhang, Jun ; Lambert, Que ; Der, Channing J. ; Del Valle, Luis ; Miklossy, Judith ; Khalili, Kamel ; Zhou, You ; Pagano, Joseph S. / Interferon regulatory factor 7 is associated with Epstein-Barr virus-transformed central nervous system lymphoma and has oncogenic properties. In: Journal of virology. 2004 ; Vol. 78, No. 23. pp. 12987-12995.
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