Interactions among subunits of human Arp2/3 complex: p20-Arc as the hub

Xiangshan Zhao, Zhenye Yang, Min Qian, Xueliang Zhu

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

The Arp2/3 complex is critical for nucleation and crosslinking of actin filaments. To gain insight into its subunit topology and assembly pathway, we systematically examined interactions among subunits of human Arp2/3 complex by yeast two-hybrid assays. It was shown that p20-Arc was able to interact with p21-Arc, p34-Arc, and p16-Arc, respectively. In contrast, p41-Arc only interacted with p20-Arc/p16-Arc heterodimer. In addition, we found that structural integrity was important for association between p20-Arc and p21-Arc, while the N-terminal half of p34-Arc was dispensable for its binding to p20-Arc. Our data suggest a key role of p20-Arc and a multistep pathway for the complex formation.

Original languageEnglish (US)
Pages (from-to)513-517
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume280
Issue number2
DOIs
StatePublished - Jan 1 2001

Fingerprint

Actin-Related Protein 2-3 Complex
Two-Hybrid System Techniques
Structural integrity
Actin Cytoskeleton
Crosslinking
Yeast
Actins
Assays
Nucleation
Topology
Association reactions

Keywords

  • Actin
  • Arp2/3 complex
  • Subunit
  • Yeast twohybrid assay

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Interactions among subunits of human Arp2/3 complex : p20-Arc as the hub. / Zhao, Xiangshan; Yang, Zhenye; Qian, Min; Zhu, Xueliang.

In: Biochemical and Biophysical Research Communications, Vol. 280, No. 2, 01.01.2001, p. 513-517.

Research output: Contribution to journalArticle

Zhao, Xiangshan ; Yang, Zhenye ; Qian, Min ; Zhu, Xueliang. / Interactions among subunits of human Arp2/3 complex : p20-Arc as the hub. In: Biochemical and Biophysical Research Communications. 2001 ; Vol. 280, No. 2. pp. 513-517.
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