Interactions among PIN-FORMED and P-glycoprotein auxin transporters in Arabidopsis

Joshua J. Blakeslee, Anindita Bandyopadhyay, Ran Lee Ok, Jozef Mravec, Boosaree Titapiwatanakun, Michael Sauer, Srinivas N. Makam, Yan Cheng, Rodolphe Bouchard, Jiří Adamec, Markus Geisler, Akitomo Nagashima, Tatsuya Sakai, Enrico Martinoia, Jiří Friml, Wendy Ann Peer, Angus S. Murphy

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282 Scopus citations

Abstract

Directional transport of the phytohormone auxin is established primarily at the point of cellular afflux and is required for the establishment and maintenance of plant polarity. Studies in whole plants and heterologous systems indicate that PIN-FORMED (PIN) and P-glycoprotein (PGP) transport proteins mediate the cellular efflux of natural and synthetic auxins. However, aromatic anion transport resulting from PGP and PIN expression in nonplant systems was also found to lack tha high level of substrate specificity seen in planta. Furthermore, previous reports that PGP19 stabilizes PIN1 on the plasma membrane suggested that PIN-PGP interactions might regulate polar auxin efflux. Hare, we show that PGP1 and PGP19 colocalized with PIN1 in the shoot apax in Arabidopsis thaliana and with PIN1 and PIN2 in root tissues. Specific PGP-PIN interactions were seen in yeast two-hybrid and colmmunoprecipitation assays. PIN-PGP interactions appeared to enhance transport activity and, to a greater extent, substrate/inhibitor specificities when coexpressed in heterologous systems. By contrast, no interactions between PGPs and the AUXIN1 influx carrier were observed. Phenotypes of pin and pgp mutants suggest discrete functional roles in auxin transport, but pin pgp mutants exhibited phenotypes that are both additive and synergistic. These results suggest that PINs and PGPs characterize coordinated, Independent auxin transport mechanisms but also function interactively in a tissue-specific manner.

Original languageEnglish (US)
Pages (from-to)131-147
Number of pages17
JournalPlant Cell
Volume19
Issue number1
DOIs
StatePublished - Jan 1 2007

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ASJC Scopus subject areas

  • Plant Science
  • Cell Biology

Cite this

Blakeslee, J. J., Bandyopadhyay, A., Ok, R. L., Mravec, J., Titapiwatanakun, B., Sauer, M., Makam, S. N., Cheng, Y., Bouchard, R., Adamec, J., Geisler, M., Nagashima, A., Sakai, T., Martinoia, E., Friml, J., Peer, W. A., & Murphy, A. S. (2007). Interactions among PIN-FORMED and P-glycoprotein auxin transporters in Arabidopsis. Plant Cell, 19(1), 131-147. https://doi.org/10.1105/tpc.106.040782