Interaction of tRNA with domain II of 23S rRNA

Walter E. Hill, Anchalee Tassanakajohn, William E. Tapprich

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The interaction of tRNA with domain II of 23S rRNA in E. coli ribosomes has been probed using short, complementary DNA oligodeoxyribonucleotides. Specifically, cDNA oligomers to the region 801-811 of the 23S rRNA were used to ascertain the interaction of this region with tRNA. It was found that when tRNA was bound to the P site, considerable competition occurred between tRNA and the cDNA oligomers which base paired with the nucleotides 807-811. However, A-site bound tRNA neither displaced, nor was displaced, by cDNA oligomers to this region. Additionally, the binding of tRNA lacking the CACCA nucleotides on the 3′ terminus was unaffected by the presence a cDNA oligomer complementary to nucleotides 803-811, indicating that the cDNA-tRNA competition was dependent on the 3′ terminal nucleotides of tRNA.

Original languageEnglish (US)
Pages (from-to)45-50
Number of pages6
JournalBBA - Gene Structure and Expression
Volume1050
Issue number1-3
DOIs
StatePublished - Aug 27 1990

Fingerprint

Transfer RNA
Complementary DNA
Oligomers
Nucleotides
Oligodeoxyribonucleotides
Ribosomes
Escherichia coli

Keywords

  • Domain II
  • rRNA, 23S
  • tRNA binding

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Genetics
  • Structural Biology

Cite this

Interaction of tRNA with domain II of 23S rRNA. / Hill, Walter E.; Tassanakajohn, Anchalee; Tapprich, William E.

In: BBA - Gene Structure and Expression, Vol. 1050, No. 1-3, 27.08.1990, p. 45-50.

Research output: Contribution to journalArticle

Hill, Walter E. ; Tassanakajohn, Anchalee ; Tapprich, William E. / Interaction of tRNA with domain II of 23S rRNA. In: BBA - Gene Structure and Expression. 1990 ; Vol. 1050, No. 1-3. pp. 45-50.
@article{f55ea7165e0543babebb27de61d0c555,
title = "Interaction of tRNA with domain II of 23S rRNA",
abstract = "The interaction of tRNA with domain II of 23S rRNA in E. coli ribosomes has been probed using short, complementary DNA oligodeoxyribonucleotides. Specifically, cDNA oligomers to the region 801-811 of the 23S rRNA were used to ascertain the interaction of this region with tRNA. It was found that when tRNA was bound to the P site, considerable competition occurred between tRNA and the cDNA oligomers which base paired with the nucleotides 807-811. However, A-site bound tRNA neither displaced, nor was displaced, by cDNA oligomers to this region. Additionally, the binding of tRNA lacking the CACCA nucleotides on the 3′ terminus was unaffected by the presence a cDNA oligomer complementary to nucleotides 803-811, indicating that the cDNA-tRNA competition was dependent on the 3′ terminal nucleotides of tRNA.",
keywords = "Domain II, rRNA, 23S, tRNA binding",
author = "Hill, {Walter E.} and Anchalee Tassanakajohn and Tapprich, {William E.}",
year = "1990",
month = "8",
day = "27",
doi = "10.1016/0167-4781(90)90139-S",
language = "English (US)",
volume = "1050",
pages = "45--50",
journal = "Biochimica et Biophysica Acta - Gene Structure and Expression",
issn = "0167-4781",
publisher = "Elsevier BV",
number = "1-3",

}

TY - JOUR

T1 - Interaction of tRNA with domain II of 23S rRNA

AU - Hill, Walter E.

AU - Tassanakajohn, Anchalee

AU - Tapprich, William E.

PY - 1990/8/27

Y1 - 1990/8/27

N2 - The interaction of tRNA with domain II of 23S rRNA in E. coli ribosomes has been probed using short, complementary DNA oligodeoxyribonucleotides. Specifically, cDNA oligomers to the region 801-811 of the 23S rRNA were used to ascertain the interaction of this region with tRNA. It was found that when tRNA was bound to the P site, considerable competition occurred between tRNA and the cDNA oligomers which base paired with the nucleotides 807-811. However, A-site bound tRNA neither displaced, nor was displaced, by cDNA oligomers to this region. Additionally, the binding of tRNA lacking the CACCA nucleotides on the 3′ terminus was unaffected by the presence a cDNA oligomer complementary to nucleotides 803-811, indicating that the cDNA-tRNA competition was dependent on the 3′ terminal nucleotides of tRNA.

AB - The interaction of tRNA with domain II of 23S rRNA in E. coli ribosomes has been probed using short, complementary DNA oligodeoxyribonucleotides. Specifically, cDNA oligomers to the region 801-811 of the 23S rRNA were used to ascertain the interaction of this region with tRNA. It was found that when tRNA was bound to the P site, considerable competition occurred between tRNA and the cDNA oligomers which base paired with the nucleotides 807-811. However, A-site bound tRNA neither displaced, nor was displaced, by cDNA oligomers to this region. Additionally, the binding of tRNA lacking the CACCA nucleotides on the 3′ terminus was unaffected by the presence a cDNA oligomer complementary to nucleotides 803-811, indicating that the cDNA-tRNA competition was dependent on the 3′ terminal nucleotides of tRNA.

KW - Domain II

KW - rRNA, 23S

KW - tRNA binding

UR - http://www.scopus.com/inward/record.url?scp=0025118160&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025118160&partnerID=8YFLogxK

U2 - 10.1016/0167-4781(90)90139-S

DO - 10.1016/0167-4781(90)90139-S

M3 - Article

C2 - 2169894

AN - SCOPUS:0025118160

VL - 1050

SP - 45

EP - 50

JO - Biochimica et Biophysica Acta - Gene Structure and Expression

JF - Biochimica et Biophysica Acta - Gene Structure and Expression

SN - 0167-4781

IS - 1-3

ER -