Interaction of the core fragments of the LL-37 host defense peptide with actin

Asaf Sol, Guangshun Wang, Edna Blotnick, Radha Golla, Gilad Bachrach, Andras Muhlrad

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Host defense peptides are effector molecules of the innate immunity that possess antimicrobial and health-promoting properties. Due to their potential therapeutic activities, host defense peptides are being explored as alternatives for antibiotics. The human LL-37 and its shorter, cost-effective, bactericidal core peptide derivates have been suggested for their therapeutic potential. Bacteria evade host defense peptides by proteolytic inactivation. Actin released from necrotized cells and abundant in infected sites was shown to bind and protect LL-37 from microbial proteolytic degradation, and to enable the peptide's antimicrobial action despite the presence of the proteases. Here, we characterized the interactions of the 10-13 residues long LL-37 core peptides with actin. We show that the LL-37 core peptides associate with actin with a lower affinity than that of LL-37. Their association with actin, which is very ionic strength sensitive, is mainly based on electrostatic interactions. Likewise, the antimicrobial activity against Escherichia coli of the minimal antimicrobial peptide KR-12 but not FK-13 nor LL-37 is also very sensitive to salts. In addition, the antimicrobial activity of the FK-13 core peptide is protected by actin against the tested bacterial proteases in a similar manner to that of LL-37, supporting its potential for therapeutic use.

Original languageEnglish (US)
Pages (from-to)9361-9367
Number of pages7
JournalRSC Advances
Volume5
Issue number13
DOIs
StatePublished - 2015

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Peptides
Actins
Peptide Hydrolases
urinary gonadotropin fragment
Antibiotics
Coulomb interactions
Ionic strength
Escherichia coli
Bacteria
Salts
Health
Association reactions
Anti-Bacterial Agents
Degradation
Molecules
Costs

ASJC Scopus subject areas

  • Chemistry(all)
  • Chemical Engineering(all)

Cite this

Sol, A., Wang, G., Blotnick, E., Golla, R., Bachrach, G., & Muhlrad, A. (2015). Interaction of the core fragments of the LL-37 host defense peptide with actin. RSC Advances, 5(13), 9361-9367. https://doi.org/10.1039/c4ra13007c

Interaction of the core fragments of the LL-37 host defense peptide with actin. / Sol, Asaf; Wang, Guangshun; Blotnick, Edna; Golla, Radha; Bachrach, Gilad; Muhlrad, Andras.

In: RSC Advances, Vol. 5, No. 13, 2015, p. 9361-9367.

Research output: Contribution to journalArticle

Sol, A, Wang, G, Blotnick, E, Golla, R, Bachrach, G & Muhlrad, A 2015, 'Interaction of the core fragments of the LL-37 host defense peptide with actin', RSC Advances, vol. 5, no. 13, pp. 9361-9367. https://doi.org/10.1039/c4ra13007c
Sol, Asaf ; Wang, Guangshun ; Blotnick, Edna ; Golla, Radha ; Bachrach, Gilad ; Muhlrad, Andras. / Interaction of the core fragments of the LL-37 host defense peptide with actin. In: RSC Advances. 2015 ; Vol. 5, No. 13. pp. 9361-9367.
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